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Publication list


2018
The Biomaker : An entry-level bioprinting device for biotechnological applications.
Radtke, C. P.; Hillebrandt, N.; Hubbuch, J.
2018. Journal of chemical technology & biotechnology, 93 (3), 792-799. doi:10.1002/jctb.5429
2017
Antibody fingerprints in lyme disease deciphered with high density peptide arrays.
Weber, L. K.; Isse, A.; Rentschler, S.; Kneusel, R. E.; Palermo, A.; Hubbuch, J.; Nesterov-Mueller, A.; Breitling, F.; Loeffler, F. F.
2017. Engineering in life sciences, 17 (10), 1078-1087. doi:10.1002/elsc.201700062
Model-Based Investigation on the Mass Transfer and Adsorption Mechanisms of Mono-Pegylated Lysozyme in Ion-Exchange Chromatography.
Morgenstern, J.; Wang, G.; Baumann, P.; Hubbuch, J. J.
2017. Biotechnology journal, 12 (9), Art.Nr.: 1700255. doi:10.1002/biot.201700255
Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution.
Palermo, A.; Weber, L. K.; Rentschler, S.; Isse, A.; Sedlmayr, M.; Herbster, K.; List, V.; Hubbuch, J.; Löffler, F. F.; Nesterov-Müller, A.; Breitling, F.
2017. Biotechnology journal, 12 (10), Art.Nr. 1700197/1-8. doi:10.1002/biot.201700197
Prediction and characterization of the stability enhancing effect of the Cherry-Tag™ in highly concentrated protein solutions by complex rheological measurements and MD simulations.
Baumann, P.; Schermeyer, M.-T.; Burghardt, H.; Dürr, C.; Gärtner, J.; Hubbuch, J.
2017. International journal of pharmaceutics, 531 (1), 360-371. doi:10.1016/j.ijpharm.2017.08.068
Root cause investigation of deviations in protein chromatography based on mechanistic models and artificial neural networks.
Wang, G.; Briskot, T.; Hahn, T.; Baumann, P.; Hubbuch, J.
2017. Journal of chromatography / A, 1515, 146-153. doi:10.1016/j.chroma.2017.07.089
Influence of the production system on the surface properties of influenza A virus particles.
Hämmerling, F.; Pieler, M. M.; Hennig, R.; Serve, A.; Rapp, E.; Wolff, M. W.; Reichl, U.; Hubbuch, J.
2017. Engineering in life sciences, 17 (10), 1071-1077. doi:10.1002/elsc.201700058
Modeling of complex antibody elution behavior under high protein load densities in ion exchange chromatography using an asymmetric activity coefficient.
Huuk, T. C.; Hahn, T.; Doninger, K.; Griesbach, J.; Hepbildikler, S.; Hubbuch, J.
2017. Biotechnology journal, 12 (3), 1600336/1-8. doi:10.1002/biot.201600336
High-throughput downstream process development for cell-based products using aqueous two-phase systems (ATPS) – A case study.
Zimmermann, S.; Scheeder, C.; Zimmermann, P. K.; Bogsnes, A.; Hansson, M.; Staby, A.; Hubbuch, J.
2017. Biotechnology journal, 12 (2), 1600587. doi:10.1002/biot.201600587
Effect of PEG molecular weight and PEGylation degree on the physical stability of PEGylated lysozyme.
Morgenstern, J.; Baumann, P.; Brunner, C.; Hubbuch, J.
2017. International journal of pharmaceutics, 519 (1-2), 408-417. doi:10.1016/j.ijpharm.2017.01.040
Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays.
Weber, L. K.; Palermo, A.; Kügler, J.; Armant, O.; Isse, A.; Rentschler, S.; Jaenisch, T.; Hubbuch, J.; Dübel, S.; Nesterov-Mueller, A.; Breitling, F.; Loeffler, F. F.
2017. Journal of immunological methods, 443, 45-54. doi:10.1016/j.jim.2017.01.012
Influence of structure properties on protein–protein interactions—QSAR modeling of changes in diffusion coefficients.
Bauer, K. C.; Hämmerling, F.; Kittelmann, J.; Dürr, C.; Görlich, F.; Hubbuch, J.
2017. Biotechnology & bioengineering, 114 (4), 821-831. doi:10.1002/bit.26210
Estimation of adsorption isotherm and mass transfer parameters in protein chromatography using artificial neural networks.
Wang, G.; Briskot, T.; Hahn, T.; Baumann, P.; Hubbuch, J.
2017. Journal of chromatography / A, 1487, 211–217. doi:10.1016/j.chroma.2017.01.068
Strategy for assessment of the colloidal and biological stability of H1N1 influenza A viruses.
Hämmerling, F.; Lorenz-Cristea, O.; Baumann, P.; Hubbuch, J.
2017. International journal of pharmaceutics, 517 (1-2), 80-87. doi:10.1016/j.ijpharm.2016.11.058
Investigation and prediction of protein precipitation by polyethylene glycol using quantitative structure–activity relationship models.
Hämmerling, F.; Ladd Effio, C.; Andris, S.; Kittelmann, J.; Hubbuch, J.
2017. Journal of biotechnology, 241, 87-97. doi:10.1016/j.jbiotec.2016.11.014
Impact of additives on the formation of protein aggregates and viscosity in concentrated protein solutions.
Bauer, K. C.; Suhm, S.; Wöll, A. K.; Hubbuch, J.
2017. International journal of pharmaceutics, 516 (1-2), 82–90. doi:10.1016/j.ijpharm.2016.11.009
Real-time monitoring and control of the load phase of a protein A capture step.
Rüdt, M.; Brestrich, N.; Rolinger, L.; Hubbuch, J.
2017. Biotechnology & bioengineering, 114 (2), 368-373. doi:10.1002/bit.26078
2016
Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts.
Buyel, J. F.; Hubbuch, J.; Fischer, R.
2016. Journal of visualized experiments, (114), Art. Nr.: e54343. doi:10.3791/54343
Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles.
Effio, C. L.; Hahn, T.; Seiler, J.; Oelmeier, S. A.; Asen, I.; Silberer, C.; Villain, L.; Hubbuch, J.
2016. Journal of chromatography, 1429, 142-154. doi:10.1016/j.chroma.2015.12.006
High-throughput process development of an alternative platform for the production of virus-like particles in Escherichia coli.
Effio, C. L.; Baumann, P.; Weigel, C.; Vormittag, P.; Middelberg, A.; Hubbuch, J.
2016. Journal of biotechnology, 219, 7-19. doi:10.1016/j.jbiotec.2015.12.018
A mechanistic model of ion-exchange chromatography on polymer fiber stationary phases.
Winderl, J.; Hahn, T.; Hubbuch, J.
2016. Journal of chromatography / A, 1475, 18–30. doi:10.1016/j.chroma.2016.10.057
Deconvolution of high-throughput multicomponent isotherms using multivariate data analysis of protein spectra.
Baumann, P.; Huuk, T.; Hahn, T.; Osberghaus, A.; Hubbuch, J.
2016. Engineering in life sciences, 16 (2), 194-201. doi:10.1002/elsc.201400243
Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification.
Hahn, T.; Huuk, T.; Osberghaus, A.; Doninger, K.; Nath, S.; Hepbildikler, S.; Heuveline, V.; Hubbuch, J.
2016. Engineering in life sciences, 16 (2), 107-113. doi:10.1002/elsc.201400248
UV absorption-based inverse modeling of protein chromatography.
Hahn, T.; Baumann, P.; Huuk, T.; Heuveline, V.; Hubbuch, J.
2016. Engineering in life sciences, 16 (2), 99-106. doi:10.1002/elsc.201400247
Implementation of an analytical microfluidic device for the quantification of protein concentrations in high-throughput format.
Radtke, C. P.; Schermeyer, M.-T.; Zhai, Y. C.; Goepper, J.; Hubbuch, J.
2016. Engineering in life sciences / Special Issue: Molecular Interaction Engineering, 16 (6), 515–524. doi:10.1002/elsc.201500185
Photoinitiated miniemulsion polymerization in microfluidic chips on automated liquid handling stations: Proof of concept.
Radtke, C. P.; Delbe, M.; Woerner, M.; Hubbuch, J.
2016. Engineering in life sciences / Special Issue: Molecular Interaction Engineering, 16 (6), 505–514. doi:10.1002/elsc.201500186
Surface tension determination by means of liquid handling stations.
Amrhein, S.; Suhm, S.; Hubbuch, J.
2016. Engineering in life sciences / Special Issue: Molecular Interaction Engineering, 16 (6), 532–537. doi:10.1002/elsc.201500179
Water on hydrophobic surfaces: Mechanistic modeling of hydrophobic interaction chromatography.
Wang, G.; Hahn, T.; Hubbuch, J.
2016. Journal of chromatography / A, 1465, 71-78. doi:10.1016/j.chroma.2016.07.085
High-throughput downstream process development for cell-based products using aqueous two-phase systems.
Zimmermann, S.; Gretzinger, S.; Schwab, M.-L.; Scheeder, C.; Zimmermann, P. K.; Oelmeier, S. A.; Gottwald, E.; Bogsnes, A.; Hansson, M.; Staby, A.; Hubbuch, J.
2016. Journal of chromatography / A, 1464, 1-11. doi:10.1016/j.chroma.2016.08.025
Concentration-dependent changes in apparent diffusion coefficients as indicator for colloidal stability of protein solutions.
Bauer, K. C.; Göbel, M.; Schwab, M.-L.; Schermeyer, M.-T.; Hubbuch, J.
2016. International journal of pharmaceutics, 511 (1), 276–287. doi:10.1016/j.ijpharm.2016.07.007
A versatile noninvasive method for adsorber quantification in batch and column chromatography based on the ionic capacity.
Huuk, T. C.; Briskot, T.; Hahn, T.; Hubbuch, J. J.
2016. Biotechnology Progress, 32 (3), 666–677. doi:10.1002/btpr.2228
Automated microfluidic system with optical setup for the inverstigation of peptide-antibody interactions in an array format.
Weber, L. K.; Fischer, A.; Schorb, T.; Soehindrijo, M.; Förtsch, T. C.; Bojnicic-Kninski, Cvon; Althuon, D.; Leffler, F. F.; Breitling, F.; Hubbuch, J.; Nesterov-Müller, A.
2016. Microsystem Technology in Germany, 50-51
High-throughput cell quantification assays for use in cell purification development - enabling technologies for cell production.
Zimmermann, S.; Gretzinger, S.; Scheeder, C.; Schwab, M.-L.; Oelmeier, S. A.; Osberghaus, A.; Gottwald, E.; Hubbuch, J.
2016. Biotechnology journal, 11 (5), 676-686. doi:10.1002/biot.201500577
Prediction of salt effects on protein phase behavior by HIC retention and thermal stability.
Baumgartner, K.; Großhans, S.; Schütz, J.; Suhm, S.; Hubbuch, J. J.
2016. Journal of pharmaceutical and biomedical analysis, 128 (Sept.), 216-225. doi:10.1016/j.jpba.2016.04.040
Impact of polymer surface characteristics on the microrheological measurement quality of protein solutions : A tracer particle screening.
Bauer, K. C.; Schermeyer, M.-T.; Seidel, J.; Hubbuch, J.
2016. International journal of pharmaceutics, 505 (1-2), 246-254. doi:10.1016/j.ijpharm.2016.03.047
Squeeze flow rheometry as a novel tool for the characterization of highly concentrated protein solutions.
Schermeyer, M.-T.; Sigloch, H.; Bauer, K. C.; Oelschlaeger, C.; Hubbuch, J.
2016. Biotechnology and Bioengineering, 113 (3), 576–587. doi:10.1002/bit.25834
2015
Advances in inline quantification of co-eluting proteins in chromatography: Process-data-based model calibration and application towards real-life separation issues.
Brestrich, N.; Sanden, A.; Kraft, A.; McCann, K.; Bertolini, J.; Hubbuch, J.
2015. Biotechnology and Bioengineering / Symposium, 112 (7), 1406–1416. doi:10.1002/bit.25546
High-throughput process development of purification alternatives for the protein avidin.
Diederich, P.; Hoffmann, M.; Hubbuch, J.
2015. Biotechnology progress, 31 (4), 957–973. doi:10.1002/btpr.2104
Predicting recombinant protein expression experiments using molecular dynamics simulation.
Schaller, A.; Connors, N. K.; Oelmeier, S. A.; Hubbuch, J.; Middelberg, A. P. J.
2015. Chemical engineering science, 121, 340-350. doi:10.1016/j.ces.2014.09.044
Cationic aerosol photopolymerization.
Akguen, E.; Muntean, A.; Hubbuch, J.; Woerner, M.; Sangermano, M.
2015. Macromolecular materials and engineering, 300 (2), 136-139. doi:10.1002/mame.201400211
A comprehensive molecular dynamics approach to protein retention modeling in ion exchange chromatography.
Lang, K. M. H.; Kittelinann, J.; Duerr, C.; Osberghaus, A.; Hubbuch, J.
2015. Journal of chromatography / A, 1381, 184-193. doi:10.1016/j.chroma.2015.01.018
Downstream processing of virus-like particles: Single-stage and multi-stage aqueous two-phase extraction.
Effio, C. L.; Wenger, L.; Oetes, O.; Oelmeier, S. A.; Kneusel, R.; Hubbuch, J.
2015. Journal of chromatography / A, 1383, 35-46. doi:10.1016/j.chroma.2015.01.007
Simulating and Optimizing Preparative Protein Chromatography with ChromX.
Hahn, T.; Huuk, T.; Heuveline, V.; Hubbuch, J.
2015. Journal of chemical education, 92 (9), 1497-1502. doi:10.1021/ed500854a
Custom-tailored adsorbers: A molecular dynamics study on optimal design of ion exchange chromatography material.
Lang, K. M. H.; Kittelmann, J.; Pilgram, F.; Osberghaus, A.; Hubbuch, J.
2015. Journal of chromatography / A, 1413, 60-67. doi:10.1016/j.chroma.2015.08.021
From osmotic second virial coefficient (B22) to phase behavior of a monoclonal antibody.
Rakel, N.; Bauer, K. C.; Galm, L.; Hubbuch, J.
2015. Biotechnology progress, 31 (2), 438-451. doi:10.1002/btpr.2065
Computational study of elements of stability of a four-helix bundle protein biosurfactant.
Schaller, A.; Connors, N. K.; Dwyer, M. D.; Oelmeier, S. A.; Hubbuch, J.; Middelberg, A. P. J.
2015. Journal of computer aided molecular design, 29 (1), 47-58. doi:10.1007/s10822-014-9803-6
High-throughput micro-scale cultivations and chromatography modeling: Powerful tools for integrated process development.
Baumann, P.; Hahn, T.; Hubbuch, J.
2015. Biotechnology and Bioengineering / Symposium, 112 (10), 2123-2133. doi:10.1002/bit.25630
Influence of macromolecular precipitants on phase behavior of monoclonal antibodies.
Rakel, N.; Galm, L.; Bauer, K. C.; Hubbuch, J. J.
2015. Biotechnology progress, 31 (1), 145-153. doi:10.1002/btpr.2027
Effect of lysozyme solid-phase PEGylation on reaction kinetics and isoform distribution.
Maiser, B.; Baumgartner, K.; Dismer, F.; Hubbuch, J.
2015. Journal of chromatography / B, 1002, 313-318. doi:10.1016/j.jchromb.2015.08.027
Integrated development of up- and downstream processes supported by the Cherry-Tag™ for real-time tracking of stability and solubility of proteins.
Baumann, P.; Bluthardt, N.; Renner, S.; Burghardt, H.; Osberghaus, A.; Hubbuch, J.
2015. Journal of biotechnology, 200, 27-37. doi:10.1016/j.jbiotec.2015.02.024
Manipulation of lysozyme phase behavior by additives as function of conformational stability.
Galm, L.; Morgenstern, J.; Hubbuch, J.
2015. International journal of pharmaceutics, 494 (1), 370-380. doi:10.1016/j.ijpharm.2015.08.045
Non-invasive high throughput approach for protein hydrophobicity determination based on surface tension.
Amrhein, S.; Bauer, K. C.; Galm, L.; Hubbuch, J.
2015. Biotechnology and Bioengineering, 112 (12), 2485-2494. doi:10.1002/bit.25677
Automatisiertes Mikrofluidic-System mit optischem Aufbau zur Untersuchung von Peptid-Antikörper-Wecselwirkungen im Arrayformat.
Weber, L. K.; Fischer, A.; Soehindrijo, M.; Förtsch, T. C.; Bojncic-Kninski, C.; Althuon, D.; Striffler, J.; Löffler, F. F.; Breitling, F.; Hubbuch, J.; Nesterov-Müller, A.
2015. MikroSystemTechnik Kongress 2015, Karlsruhe, 26.-28.Oktober 2015
Automatisiertes Mikrofluidic-System mit optischem Aufbau zur Untersuchung von Peptid-Antikörper-Wecselwirkungen im Arrayformat.
Weber, L. K.; Fischer, A.; Soehindrijo, M.; Förtsch, T. C.; Bojncic-Kninski, C.; Althuon, D.; Striffler, J.; Löffler, F. F.; Breitling, F.; Hubbuch, J.; Nesterov-Müller, A.
2015. MikroSystemTechnik Kongress 2015, Karlsruhe, 26.-28.Oktober 2015, 120-122, VDE-Verlag, Berlin
Light extinction and scattering by agarose based resin beads and applications in high-throughput screening.
Kittelmann, J.; Hämmerling, F.; Ebeler, M.; Hubbuch, J.
2015. Journal of Chromatography A, 1397, 52-58. doi:10.1016/j.chroma.2015.04.013
2014
Defined polymer shells on nanoparticles via a continuous aerosol-based process.
Sigmund, S.; Akgün, E.; Meyer, J.; Hubbuch, J.; Wörner, M.; Kasper, G.
2014. Journal of Nanoparticle Research, 16 (8), 2533. doi:10.1007/s11051-014-2533-9
Determination of protein phase diagrams by microbatch experiments: Exploring the influence of precipitants and pH.
Baumgartner, K.; Galm, L.; Nötzold, J.; Sigloch, H.; Morgenstern, J.; Schleining, K.; Suhm, S.; Oelmeier, S. A.; Hubbuch, J.
2014. International Journal of Pharmaceutics, 479 (1), 28-40. doi:10.1016/j.ijpharm.2014.12.027
A tool for selective inline quantification of co-eluting proteins in chromatography using spectral analysis and partial least squares regression.
Brestrich, N.; Briskot, T.; Osberghaus, A.; Hubbuch, J.
2014. Biotechnology and Bioengineering, 111 (7), 1365-373. doi:10.1002/bit.25194
Model-based integrated optimization and evaluation of a multi-step ion exchange chromatography.
Huuk, T.; Hahn, T.; Osberghaus, A.; Hubbuch, J.
2014. Separation and Purification Technology, 136, 207-222. doi:10.1016/j.seppur.2014.09.012
Meeting Report VLPNPV: Session 10: Virus-like particle and nano-particle vaccines.
Ladd Effio, C.; Hubbuch, J.
2014. Human Vaccines & Immunotherapeutics, 10 (10), 3080-3082. doi:10.4161/hv.29840
Untersuchung des Einflusses des Beschichtungsprozesses auf die Aktivität eines eingebetteten Enzyms in gedruckten Biosensoren.
Riegel, A. L.; Borzenkova, N.; Hubbuch, J.; Scharfer, P.; Schabel, W.
2014. Chemie - Ingenieur - Technik, 86 (9), 1477. doi:10.1002/cite.201450411
Soluble full-length expression and characterization of snRNP protein U1-68/70 K.
Richter, C.; Simon, T.; Asen, I.; Brenn-Weiss, G.; Hubbuch, J.
2014. Protein expression and purification, 104, 65-70. doi:10.1016/j.pep.2014.08.009
Microfluidics on liquid handling stations (μF-on-LHS): A new industry-compatible microfluidic platform.
Kittelmann, J.; Radtke, C. P.; Waldbaur, A.; Neumann, C.; Hubbuch, J.; Rapp, B. E.
2014. Microfluidics, BioMEMS, and Medical Microsystems XII : Proceedings of Photonics West, San Francisco, Calif., February 2-4, 2014. Ed.: B. L. Gray, Article no 89760G, SPIE, Bellingham, WA. doi:10.1117/12.2044665
Microfluidics on liquid handling stations (μF-on-LHS): A new industry-compatible microfluidic platform.
Waldbaur, A.; Kittelmann, J.; Radtke, C. P.; Hubbuch, J.; Rapp, B. E.
2014. Microfluidics, BioMEMS, and Medical Microsystems XII, San Francisco, Calif., February 2-4, 2014
2013
Microfluidics on liquid handling stations (μF-on-LHS): an industry compatible chip interface between microfluidics and automated liquid handling stations.
Waldbaur, A.; Kittelmann, J.; Radtke, C. P.; Hubbuch, J.; Rapp, B. E.
2013. Lab on a Chip - Miniaturisation for Chemistry and Biology, 13 (12), 2337-2343. doi:10.1039/c3lc00042g
2012
Model-integrated process development demonstrated on the optimization of a robotic cation exchange step.
Osberghaus, A.; Drechsel, K.; Hansen, S.; Hepbildikler, S.; Nath, S.; Haindl, M.; Lieres, E. von; Hubbuch, J.
2012. Chemical Engineering Science, 76, 129-139. doi:10.1016/j.ces.2012.04.004
Examination of a genetic algorithm for the application in high-throughput downstream process development.
Treier, K.; Berg, A.; Diederich, P.; Lang, K.; Osberghaus, A.; Dismer, F.; Hubbuch, J.
2012. Biotechnology Journal, 7 (10), 1203-1215. doi:10.1002/biot.201200145
Detection, quantification, and propagation of uncertainty in high-throughput experimentation by Monte Carlo methods.
Osberghaus, A.; Baumann, P.; Hepbildikler, S.; Nath, S.; Haindl, M.; Lieres, E. von; Hubbuch, J.
2012. Chemical Engineering and Technology, 35 (8), 1456-1464. doi:10.1002/ceat.201100610
Determination of parameters for the steric mass action model - A comparison between experimental and modeling approaches.
Osberghaus, A.; Hepbildikler, S.; Nath, S.; Haindl, M.; Lieres, E. von; Hubbuch, J.
2012. Journal of Chromatography A, 1233, 54-64. doi:10.1016/j.chroma.2012.02.004
Optimizing a chromatographic three component separation: A comparison of mechanistic and empiric modeling approaches.
Osberghaus, A.; Hepbildikler, S.; Nath, S.; Haindl, M.; Lieres, E. von; Hubbuch, J.
2012. Journal of Chromatography A, 1237, 86-95. doi:10.1016/j.chroma.2012.03.029
Isoform separation and binding site determination of mono-PEGylated lysozyme with pH gradient chromatography.
Maiser, B.; Kröner, F.; Dismer, F.; Brenner-Weiß, G.; Hubbuch, J.
2012. Journal of Chromatography A, 1268, 102-108. doi:10.1016/j.chroma.2012.10.047
2011
A label-free methodology for selective protein quantification by means of absorption measurements.
Hansen, S. K.; Skibsted, E.; Staby, A.; Hubbuch, J.
2011. Biotechnology and Bioengineering, 108 (11), 2661-2669. doi:10.1002/bit.23229
2010
Application of an aqueous two-phase systems high-throughput screening method to evaluate mAb HCP separation.
Oelmeier, S. A.; Dismer, F.; Hubbuch, J.
2010. Biotechnology and Bioengineering, 108 (1), 69-81. doi:10.1002/bit.22900
3D structure-based protein retention prediction for ion-exchange chromatography.
Dismer, F.; Hubbuch, J.
2010. Journal of Chromatography A, 1217 (8), 1343-1354. doi:10.1016/j.chroma.2009.12.061
2009
High Throughput Screening for the Design and Optimization of Chromatographic Processes: Automated Optimization of Chromatographic Phase Systems.
Susanto, A.; Treier, K.; Knieps-Grünhagen, E.; von Lieres, E.; Hubbuch, J.
2009. Chemical Engineering & Technology, 32 (1), 140-154. doi:10.1002/ceat.200800350
Rational and systematic protein purification process development: the next generation.
Nfor, B. K.; Verhaert, P. D. E. M.; van der Wielen, L. A. M.; Hubbuch, J.; Ottens, M.
2009. Trends in Biotechnology, 27 (12), 673-679. doi:10.1016/j.tibtech.2009.09.002
2008
Detailed analysis of membrane adsorber pore structure and protein binding by advanced microscopy.
Wang, J.; Dismer, F.; Hubbuch, J.; Ulbricht, M.
2008. Journal of Membrane Science, 320 (1-2), 456-467. doi:10.1016/j.memsci.2008.04.039
Critical evaluation and comparison of fluid distribution systems for industrialscale expanded bed adsorption chromatography columns.
Arpanaei, A.; Heebøll-Nielsen, A.; Hubbuch, J. J.; Thomas, O. R. T.; Hobley, T. J.
2008. Journal of Chromatography A, 1198-1199, 131-139. doi:10.1016/j.chroma.2008.05.044
High Throughput Screening for the Design and Optimization of Chromatographic Processes - Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies.
Wiendahl, M.; Schulze Wierling, P.; Nielsen, J.; Fomsgaard Christensen, D.; Krarup, J.; Staby, A.; Hubbuch, J.
2008. Chemical Engineering & Technology, 31 (6), 893-903. doi:10.1002/ceat.200800167
Confocal laser scanning microscopy as an analytical tool in chromatographic research.
Hubbuch, J.; Kula, M. R.
2008. Bioprocess and Biosystems Engineering, 31 (3), 241-259
2007
Comparison of chromatographic ion-exchange resins VI. Weak anion-exchange resins.
Staby, A.; Jensen, R. H.; Bensch, M.; Hubbuch, J.; Dunweber, D. L.; Krarup, J.; Nielsen, J.; Lund, M.; Kidal, S.; Hansen, T. B.; Jensen, I. H.
2007. Journal of Chromatography A, 1164 (1-2), 82-94. doi:10.1016/j.chroma.2007.06.048
Isolation and purification of biotechnological products.
Hubbuch, J.; Kula, M. R.
2007. Journal of Non-Equilibrium Thermodynamics, 32, 99-127
High throughput screening of packed bed chromatography coupled with SELDI-TOF MS analysis: Monoclonal antibodies vs. host cell protein.
Wierling, P. S.; Bogumil, R.; Knieps-Grunhagen, E.; Hubbuch, J.
2007. Biotechnology and Bioengineering, 98 (2), 440-450. doi:10.1002/bit.21399
2006
Characterizing solute binding to macroporous ion exchange membrane adsorbers using confocal laser scanning microscopy.
Wickramasinghe, S. R.; Carlson, J. O.; Teske, C.; Hubbuch, J.; Ulbricht, M.
2006. Journal of Membrane Science, 281 (1-2), 609-618. doi:10.1016/j.memsci.2006.04.032
Competitive adsorption of labeled and native protein in confocal laser scanning microscopy.
Teske, C. A.; Lieres, E. von; Schröder, M.; Ladiwala, A.; Cramer, S. M.; Hubbuch, J. J.
2006. Biotechnology and Bioengineering, 95 (1), 58-66. doi:10.1002/bit.20940
Generation of equally sized particles using solid-liquid suspensions.
Herrmann, T.; Schroder, M.; Hubbuch, J.
2006. Biotechnology Progress, 22 (3), 914-918. doi:10.1021/bp050296i
The influence of homogenisation conditions on biomass-adsorbent interactions during ion-exchange expanded bed adsorption.
Hubbuch, J. J.; Brixius, P. J.; Lin, D.-Q.; Mollerup, I.; Kula, M.-R.
2006. Biotechnology and Bioengineering, 94 (3), 543-553. doi:10.1002/bit.20850
Direct quantification of intra-particle protein diffusion in chromatographic media.
Schroder, M.; Lieres, E. von; Hubbuch, J.
2006. Journal of Physical Chemistry B, 110 (3), 1429-1436. doi:10.1021/jp0542726
2005
High throughput screening of chromatographic phases for rapid process development.
Bensch, M.; Wierling, P. S.; Lieres, E. von; Hubbuch, J.
2005. Chemical Engineering & Technology, 28 (11), 1274-1284. doi:10.1002/ceat.200500153
Scalable recovery of plasmid DNA based on aqueous two-phase separations.
Frerix, A.; Muller, M.; Kula, M. R.; Hubbuch, J.
2005. Biotechnology and Applied Biochemistry, 42 (1), 57-66
Protein labelling effects in confocal laser scanning microscopy.
Teske, C. A.; Schroeder, M.; Simon, R.; Hubbuch, J.
2005. Journal of Physical Chemistry B, 109 (28), 13811-13817. doi:10.1021/jp050713+
Integrated bioprocesses.
Schugerl, K.; Hubbuch, J.
2005. Current Opinion in Microbiology, 8 (3), 294-300. doi:10.1016/j.mib.2005.01.002
Biochemical Engineering Aspects of Expanded Bed Adsorption.
Hubbuch, J.; Thömmes, J.; Kula, M.-R.
2005. Advances in biochemical engineering, biotechnology, 92, 101-123
2004
The influence of biomass on the hydrodynamic behaviour and stability of expanded beds.
Lin, D.-Q.; Thömmes, J.; Kula, M.-R.; Hubbuch, J. J.
2004. Biotechnology and Bioengineering, 87 (3), 337-346. doi:10.1002/bit.20118
Superparamagnetic adsorbents for high-gradient magnetic fishing of lectins out of legume extracts.
Heebøll-Nielsen, A.; Dalkiær, M.; Hubbuch, J. J.; Thomas, O. R. T.
2004. Biotechnology and Bioengineering, 87 (3), 311-323. doi:10.1002/bit.20116
2003
Mechanism and dynamics of protein transport in chromatographic media studied by confocal laser scanning microscopy. Part 2: Impact on chromatographic separations.
Hubbuch, J.; Linden, T.; Knieps, E.; Ljunglof, A.; Thommes, J.; Kula, M. R.
2003. Journal of Chromatography A, 1021 (1-2), 105-115. doi:10.1016/j.chroma.2003.08.092
Biomass/adsorbent electrostatic interactions in expanded bed adsorption: a zeta potential study.
Lin, D. Q.; Brixius, P. J.; Hubbuch, J. J.; Thommes, J.; Kula, M. R.
2003. Biotechnology and Bioengineering, 83 (2), 149-157. doi:10.1002/bit.10654
2002
Dynamics of protein uptake within the adsorbent particle during packed bed chromatography.
Hubbuch, J.; Linden, T.; Knieps, E.; Thommes, J.; Kula, M. R.
2002. Biotechnology and Bioengineering, 80 (4), 359-368. doi:10.1002/bit.10500
High gradient magnetic affinity separation of trypsin from porcine pancreatin.
Hubbuch, J. J.; Thomas, O. R. T.
2002. Biotechnology and Bioengineering, 79 (3), 301-313. doi:10.1002/bit.10285
A New Fluid Distribution System for Scale-Flexible Expanded Bed Adsorption.
Hubbuch, J. J.; Heeboll-Nielsen, A.; Hobley, T. J.; Thomas, O. R. T.
2002. Biotechnology and Bioengineering, 78 (1), 35-43. doi:10.1002/bit.10170
2001
The Use of Ion-Selective Electrodes for Evaluating Residence Time Distributions in Expanded Bed Adsorption Systems.
Fernandez-Lahore, H. M.; Lin, D. Q.; Hubbuch, J. J.; Kula, M. R.; Thommes, J.
2001. Biotechnology Progress, 17 (6), 1128-1136. doi:10.1021/bp010118z
High gradient magnetic separation versus expanded bed adsorption: A first principle comparison.
Hubbuch, J. J.; Matthiesen, D. B.; Hobley, T. J.; Thomas, O. R. T.
2001. Bioseparation, 10 (1-3), 99112