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Nicolai_Bluthardt_2018

M.Sc. Nicolai Bluthardt

Engineer
room: R. 209
phone: +49 721 608 47525
nicolai bluthardtZmu3∂kit edu

Fritz-Haber-Weg 2

Gebäude 30.44

76131 Karlsruhe



CV and Publications

Scientific CV

09/2017 – now Research associate at Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
09/2016 – 03/2017 Master thesis
  Alternatives to protein PEGylation Stability assessment of conjugated proteins
  Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
04/2014 – 09/2017 Research assistant
  Cultivation of E.Coli (BioLector System)
  Cell disruption (ultrasonic)
  Downstream processing (affinity chromatography, IEX, HIC)
  Analytics (capillary gel electrophoresis, SEC, MALDI-TOF, activity assays, OPTIM, NMR)
10/2015 – 02/2016 Internship at Synthon, Nijmegen
  Development of a chromatographic step to separate Antibody Drug Conjugate Species
11/2013 – 03/2014 Bachelor thesis
  Optimization of the production of soluble and native cherry-tag proteins using a high throughput approach
  Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
10/2010 – 03/2017 Studies of Bioengineering at Karlsruhe Institute of Technology (KIT)



 

Research Projects

Biopharmaceutical downstream processing, industry projects



 

Publications

Papers

P. Baumann, N. Bluthardt, S. Renner, H. Burghardt, A. Osberghaus, J. Hubbuch, Integrated development of up- and downstream processes supported by the Cherry-Tag for real-time tracking of stability and solubility of proteins, J. Biotechnol. 200 (2015) 27-37, http://dx.doi.org/10.1016/j.jbiotec.2015.02.024
 

Lebenslauf und Publikationen

Akademischer CV

09/2017 - jetzt Ingenieur am Institut für Bio- und Lebensmitteltechnik, Molekulare Aufarbeitung von Bioprodukten, KIT
09/2016 – 03/2017 Masterarbeit
  Alternatives to protein PEGylation Stability assessment of conjugated proteins
  Institut für Bio- und Lebensmitteltechnik, Molekulare Aufarbeitung von Bioprodukten, KIT
04/2014 – 09/2017 Wissenschaftliche Hilfskraft
  Kultivierung von E.Coli (BioLector System)
  Zellaufschluss (Ultraschall)
  Aufreinigung (Affinitätschromatographie, IEX, HIC)
  Analytik (Kapillargelelektrophorese, SEC, MALDI-TOF, Aktivitätsassays, OPTIM, NMR)
10/2015 – 02/2016 Praktikum bei Synthon, Nijmegen
  Development of a chromatographic step to separate Antibody Drug Conjugate Species
11/2013 – 03/2014 Bachelorarbeit
  Optimization of the production of soluble and native cherry-tag proteins using a high throughput approach
  Institut für Bio- und Lebensmitteltechnik, Molekulare Aufarbeitung von Bioprodukten, KIT
10/2010 – 03/2017 Studium des Bioingenieurwesens am Karlsruher Institut für Technologie (KIT)



 

Forschungsbreich

Biopharmazeutische Aufreinigung, Industrieprojekte



 

Publikationen

Artikel

P. Baumann, N. Bluthardt, S. Renner, H. Burghardt, A. Osberghaus, J. Hubbuch, Integrated development of up- and downstream processes supported by the Cherry-Tag for real-time tracking of stability and solubility of proteins, J. Biotechnol. 200 (2015) 27-37, http://dx.doi.org/10.1016/j.jbiotec.2015.02.024