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Stefan

Dr.-Ing. Stefan Oelmeier

Post Doc
office hours: Donnerstags 13:00-14:00 nach Anmeldung (Email oder ILIAS)
room: R. 020
phone: +49 721 608 46227
fax: +49 721 608 46240
stefan oelmeierUre2∂kit edu

Engler-Bunte-Ring 3
Gebäude 40.12
76131 Karlsruhe


Resume and publications

Scientific CV

06/2012 - now

PostDoc at the Karlsruhe Institute of Technology

06/2008 - 06/2012
Graduate Student at the University of Karlsruhe

07/2007 - 05/2008

Masterthesis „An evaluation of displacement chromatography for the manufacturing scale purification of a complex fusion protein“

Process Biochemistry Group, BiogenIdec Inc., San Diego, USA

10/2005 - 06/2007

Study of „Biotechnology / molecular biotechnology“ M. Sc. at the RWTH Aachen University

08/2005 - 10/2005

Internship „Characterisation of allergens transiently expressed in tabacco leaves“

Institute for molecular biotechnology at the RWTH Aachen University

04/2005 - 07/2005

Bachelorthesis „Characterization of the promoter and first gene of the phe-operon of Geobacillus stearothermophilus“

Institute for biotechnology at the RWTH Aachen University

10/2002 - 06/2005

Study of „Biotechnology / molecular biotechnology“ B.Sc. at the RWTH Aachen University

Research Project

Current

Protein-protein interactions, focussed on protein phase diagrams, protein crystallization and precipitation, and properties of highly concentrated protein solution. Purification of large biological entities (mammalian cells, VLPs) using non-chromatographic processes. Molecular dynamics simutions of aqueous two-phase systems.

Dissertation

Evaluation of high-throughput screening methologies for the determination of solubility and partitioning in aqueous two-phase systems of proteins.

Publications

Poster

Stefan Oelmeier, Jürgen Hubbuch
Screening Aqueous Two Phase Systems for the Purification of Proteins using a Robotic Platform
15th International Conference on Biopartitioning and Purification (BPP)
Brunel University, Uxbridge, UK (14-19 June 2009)

Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch
Characterizing phase formation in aqueous two phase systems by molecular dynamics simulations
International Symposium on the Separation of Proteins, Peptides & Polynucleotides – Bologna – 2010

Stefan Oelmeier, Jürgen Hubbuch
Statistical Profling of an automated screening method in the case of an ATPS screening tool
1st International Conference on High-Throughput Process Development – Krakow – 2010

Florian Dismer, Stefan Oelmeier, Jürgen Hubbuch
Robotics aided solvatochromic characterization of PEG-PO4 ATPS
16th International Conference on BioPartitioning and Purifcation - Puerto Vallarta – 2011

Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch
Evaluation of Centrifugal Partitioning Chromatography for the purifcation of monoclonal antibodies
16th International Conference on BioPartitioning and Purifcation - Puerto Vallarta – 2011

Talks

Florian Dismer, Stefan Oelmeier, Jürgen Hubbuch
Gaining mechanistic understanding of aqueous two-phase systems for bioseparation
1st European Congress of Applied Biotechnology – Berlin – 2011

 

Stefan Oelmeier, Jürgen Hubbuch
High throughput analytics
BioProcess International conference – Long Beach, CA, USA – 2011

Stefan Oelmeier, Jürgen Hubbuch
Protein Partitioning in Aqueous Two Phase Systems: A Molecular Dynamics Approach
ACS Conference, April 2012, San Diego, USA

 

Oelmeier, SA, Dismer, F, Dieterle, M, Richter, M, Hubbuch, J
Process Development Balancing Solubility and Partitioning –Aqueous Two-Phase Extraction of Proteins
July 29.-August 3. 2012, Recovery of Biological Products 15, Stowe, USA

 

Diederich P., Hansen S.K., Oelmeier S.A., Hubbuch J.
MAb-Aggregate Analysis within 2 Minutes using Parallel-Interlaced HPLC
Bioprocessing Summit, August 20-23, Boston, MA,USA

Paper

 

Alternative separation steps for monoclonal antibody purification: Combination of centrifugal partitioning chromatography and precipitation

 

High throughput screening based selection of phases for aqueous two-phase system-centrifugal partitioning chromatography of monoclonal antibodies

 

Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch (2011)
Application of an aqueous two-phase systems high-throughput screening method to evaluate mAb HCP separation.
Biotechnology and bioengineering, 108(1), pp. 69-81 - link


Patrick Diederich, Sigrid K. Hansen, Stefan A. Oelmeier, Bianca Stolzenberger, Jürgen Hubbuch (2011)
A Sub-Two Minutes Method for Monoclonal Antibody-Aggregate Quantification using Parallel Interlaced Size Exclusion High Performance Liquid Chromatography
J Chrom A, 1218, pp. 9010-8 - link


Berg, A., Oelmeier, S. A., Kittelmann, J., Dismer, F., & Hubbuch, J. (2012)
Development and characterization of an automated high throughput screening method for optimization of protein refolding processes
Journal of separation science, 35(22), 3149–59 - link


Oelmeier, S. A., Dismer, F., & Hubbuch, J. (2012)
Molecular dynamics simulations on aqueous two-phase systems - Single PEG-molecules in solution
BMC biophysics, 5, 14 - link


Oelmeier, S. A., Ladd Effio, C., & Hubbuch, J. (2012)
High throughput screening based selection of phases for aqueous two-phase system-centrifugal partitioning chromatography of monoclonal antibodies
Journal of chromatography. A, 1252, 104–14 - link


Wiendahl, M., Oelmeier, S. A., Dismer, F., & Hubbuch, J. (2012)
High-throughput screening-based selection and scale-up of aqueous two-phase systems for pDNA purification
Journal of separation science, 35(22), 3197–207 - link


Hansen, S. K., & Oelmeier, S. A. (2012)
Meeting report: High-throughput process development--HTPD 2012: June 4-7, 2012, Avignon, France
Biotechnology journal, 7(10), 1189–91 - link


Dismer, F., Oelmeier, S. A., & Hubbuch, J. (2013) Molecular dynamics simulations of aqueous two-phase systems: Understanding phase formation and protein partitioning.
Chemical Engineering Science, 96, 142–151 - link


Dismer, F., Hansen, S., Oelmeier, S. A., & Hubbuch, J. (2013)
Accurate retention time determination of co-eluting proteins in analytical chromatography by means of spectral data.
Biotechnology and bioengineering, 110(3), 683–93 - link


Schulte, B., John, I., Simon, B., Brockmann, C., Oelmeier, S. A, Jahraus, B., Kirchgessner, H., Riplinger, S., Carlomagno, T., Wabnitz, G., Samstag, Y. (2013)
A reducing milieu renders cofilin insensitive to phosphatidylinositol 4,5-bisphosphate (PIP2) inhibition
The Journal of biological chemistry - link