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Stefan

Dr.-Ing. Stefan Oelmeier

Postdoc
Sprechstunden: Donnerstags 13:00-14:00 nach Anmeldung (Email oder ILIAS)
Raum: R. 020
Tel.: +49 721 608 46227
Fax: +49 721 608 46240
stefan oelmeierTzh9∂kit edu

Engler-Bunte-Ring 3
Gebäude 40.12
76131 Karlsruhe


Lebenslauf und Publikationen

Wissenschaftlicher Lebenslauf

06/2012 - jetzt

PostDoc am Karlsruhe Institut für Technologie

06/2008 - 06/2012

Doktorand an der Universität Karlsruhe

07/2007 - 05/2008

Masterarbeit „An evaluation of displacement chromatography for the manufacturing scale purification of a complex fusion protein“

Process Biochemistry Group, BiogenIdec Inc., San Diego, USA

10/2005 - 06/2007

Masterstudium „Biotechnologie / Molekulare Biotechnologie“ M. Sc. an der RWTH Aachen

08/2005 - 10/2005

Forschungspraktikum „Charakterisierung transient in Tabak expremierter Pollenallergene“

Institut für Molekulare Biotechnologie an der RWTH Aachen

04/2005 - 07/2005

Bachelorarbeit „Characterization of the promoter and first gene of the phe-operon of Geobacillus stearothermophilus“

Institut für Biotechnologie an der RWTH Aachen

10/2002 - 06/2005

Bachelorstudium „Biotechnologie / Molekulare Biotechnologie“ an der RWTH Aachen

Forschungsbereich

Aktuell

Arbeiten zur Proteinlöslichkeit, Phasendiagrammen und die Auswirkung von Protein-Protein Wechselwirkungen auf die Lösungscharakteristika. Aufreinigung komplexer biologischer Stoffe (Zellen, VLPs) über nicht-chromatographische Verfahren. Moleküldynamische Untersuchungen zu wässrigen Zweiphasensystemen.

Promotion

Evaluierung von Hochdurchsatzverfahren zur Löslichkeitsbestimmung und zur wässrigen Zweiphasenextraktion von Proteinen

Publikationen

Poster

Stefan Oelmeier, Jürgen Hubbuch
Screening Aqueous Two Phase Systems for the Purification of Proteins using a Robotic Platform
15th International Conference on Biopartitioning and Purification (BPP)
Brunel University, Uxbridge, UK (14-19 June 2009)

Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch
Characterizing phase formation in aqueous two phase systems by molecular dynamics simulations
International Symposium on the Separation of Proteins, Peptides & Polynucleotides – Bologna – 2010

Stefan Oelmeier, Jürgen Hubbuch
Statistical Profling of an automated screening method in the case of an ATPS screening tool
1st International Conference on High-Throughput Process Development – Krakow – 2010

Florian Dismer, Stefan Oelmeier, Jürgen Hubbuch
Robotics aided solvatochromic characterization of PEG-PO4 ATPS
16th International Conference on BioPartitioning and Purifcation - Puerto Vallarta – 2011

Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch
Evaluation of Centrifugal Partitioning Chromatography for the purifcation of monoclonal antibodies
16th International Conference on BioPartitioning and Purifcation - Puerto Vallarta – 2011

Talks

Florian Dismer, Stefan Oelmeier, Jürgen Hubbuch
Gaining mechanistic understanding of aqueous two-phase systems for bioseparation
1st European Congress of Applied Biotechnology – Berlin – 2011

 

Stefan Oelmeier, Jürgen Hubbuch
High throughput analytics
BioProcess International conference – Long Beach, CA, USA – 2011

Stefan Oelmeier, Jürgen Hubbuch
Protein Partitioning in Aqueous Two Phase Systems: A Molecular Dynamics Approach
ACS Conference, April 2012, San Diego, USA

 

Oelmeier, SA, Dismer, F, Dieterle, M, Richter, M, Hubbuch, J
Process Development Balancing Solubility and Partitioning –Aqueous Two-Phase Extraction of Proteins
July 29.-August 3. 2012, Recovery of Biological Products 15, Stowe, USA

 

Diederich P., Hansen S.K., Oelmeier S.A., Hubbuch J.
MAb-Aggregate Analysis within 2 Minutes using Parallel-Interlaced HPLC
Bioprocessing Summit, August 20-23, Boston, MA,USA

Paper

Alternative separation steps for monoclonal antibody purification: Combination of centrifugal partitioning chromatography and precipitation

High throughput screening based selection of phases for aqueous two-phase system-centrifugal partitioning chromatography of monoclonal antibodies

 

Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch (2011)
Application of an aqueous two-phase systems high-throughput screening method to evaluate mAb HCP separation.
Biotechnology and bioengineering, 108(1), pp. 69-81 - link


Patrick Diederich, Sigrid K. Hansen, Stefan A. Oelmeier, Bianca Stolzenberger, Jürgen Hubbuch (2011)
A Sub-Two Minutes Method for Monoclonal Antibody-Aggregate Quantification using Parallel Interlaced Size Exclusion High Performance Liquid Chromatography
J Chrom A, 1218, pp. 9010-8 - link


Berg, A., Oelmeier, S. A., Kittelmann, J., Dismer, F., & Hubbuch, J. (2012)
Development and characterization of an automated high throughput screening method for optimization of protein refolding processes
Journal of separation science, 35(22), 3149–59 - link


Oelmeier, S. A., Dismer, F., & Hubbuch, J. (2012)
Molecular dynamics simulations on aqueous two-phase systems - Single PEG-molecules in solution
BMC biophysics, 5, 14 - link


Oelmeier, S. A., Ladd Effio, C., & Hubbuch, J. (2012)
High throughput screening based selection of phases for aqueous two-phase system-centrifugal partitioning chromatography of monoclonal antibodies
Journal of chromatography. A, 1252, 104–14 - link


Wiendahl, M., Oelmeier, S. A., Dismer, F., & Hubbuch, J. (2012)
High-throughput screening-based selection and scale-up of aqueous two-phase systems for pDNA purification
Journal of separation science, 35(22), 3197–207 - link


Hansen, S. K., & Oelmeier, S. A. (2012)
Meeting report: High-throughput process development--HTPD 2012: June 4-7, 2012, Avignon, France
Biotechnology journal, 7(10), 1189–91 - link


Dismer, F., Oelmeier, S. A., & Hubbuch, J. (2013) Molecular dynamics simulations of aqueous two-phase systems: Understanding phase formation and protein partitioning.
Chemical Engineering Science, 96, 142–151 - link


Dismer, F., Hansen, S., Oelmeier, S. A., & Hubbuch, J. (2013)
Accurate retention time determination of co-eluting proteins in analytical chromatography by means of spectral data.
Biotechnology and bioengineering, 110(3), 683–93 - link


Schulte, B., John, I., Simon, B., Brockmann, C., Oelmeier, S. A, Jahraus, B., Kirchgessner, H., Riplinger, S., Carlomagno, T., Wabnitz, G., Samstag, Y. (2013)
A reducing milieu renders cofilin insensitive to phosphatidylinositol 4,5-bisphosphate (PIP2) inhibition
The Journal of biological chemistry - link