Publikationsliste

Effective removal of host cell-derived nucleic acids bound to hepatitis B core antigen virus-like particles by heparin chromatography
Valentic, A.; Hubbuch, J.
2024. Frontiers in Bioengineering and Biotechnology, 12, 1475918. doi:10.3389/fbioe.2024.1475918

Mechanistic modeling of minute virus of mice surrogate removal by anion exchange chromatography in micro scale
Döring, L.; Winderl, J.; Kron, M.; Hubbuch, J.
2024. Journal of Chromatography A, 1734, 465261. doi:10.1016/j.chroma.2024.465261

RP-CAD for Lipid Quantification: Systematic Method Development and Intensified LNP Process Characterization
Beckert, N.; Dietrich, A.; Hubbuch, J.
2024. Pharmaceuticals, 17 (9), 1217. doi:10.3390/ph17091217

Partitioning behavior of short DNA fragments in polymer/salt aqueous two‐phase systems
Meutelet, R.; Bisch, L. J.; Buerfent, B. C.; Müller, M.; Hubbuch, J.
2024. Biotechnology Journal, 19 (9), Art.-Nr.: 2400394. doi:10.1002/biot.202400394

An HPLC-SEC-based rapid quantification method for vesicular stomatitis virus particles to facilitate process development
Schimek, A.; Ng, J. K. M.; Basbas, I.; Martin, F.; Xin, D.; Saleh, D.; Hubbuch, J.
2024. Molecular Therapy - Methods & Clinical Development, 32 (2), Art.-Nr.: 101252. doi:10.1016/j.omtm.2024.101252

Raman-based PAT for VLP precipitation: systematic data diversification and preprocessing pipeline identification
Dietrich, A.; Schiemer, R.; Kurmann, J.; Zhang, S.; Hubbuch, J.
2024. Frontiers in Bioengineering and Biotechnology, 12, Art.-Nr.: 1399938. doi:10.3389/fbioe.2024.1399938

Spectroscopic insights into multi-phase protein crystallization in complex lysate using Raman spectroscopy and a particle-free bypass
Wegner, C. H.; Eming, S. M.; Walla, B.; Bischoff, D.; Weuster-Botz, D.; Hubbuch, J.
2024. Frontiers in Bioengineering and Biotechnology, 12, Art.-Nr. 1397465. doi:10.3389/fbioe.2024.1397465

Predicting multimodal chromatography of therapeutic antibodies using multiscale modeling
Hess, R.; Faessler, J.; Yun, D.; Mama, A.; Saleh, D.; Grosch, J.-H.; Wang, G.; Schwab, T.; Hubbuch, J.
2024. Journal of Chromatography A, 1718, Art.-Nr.: 464706. doi:10.1016/j.chroma.2024.464706

Kinetic models towards an enhanced understanding of diverse ADC conjugation reactions
Weggen, J. T.; Bean, R.; Hui, K.; Wendeler, M.; Hubbuch, J.
2024. Frontiers in Bioengineering and Biotechnology, 12, Art.-Nr.: 1403644. doi:10.3389/fbioe.2024.1403644

Generative data augmentation and automated optimization of convolutional neural networks for process monitoring
Schiemer, R.; Rüdt, M.; Hubbuch, J.
2024. Frontiers in Bioengineering and Biotechnology, 12, Art.-Nr.: 1228846. doi:10.3389/fbioe.2024.1228846

Absolute Quantification of Hepatitis B Core Antigen (HBcAg) Virus-like Particles and Bound Nucleic Acids
Valentic, A.; Böhner, N.; Hubbuch, J.
2024. Viruses, 16 (1), Art.-Nr.: 13. doi:10.3390/v16010013

On the reproducibility of extrusion-based bioprinting: round robin study on standardization in the field
Grijalva Garces, D.; Strauß, S.; Gretzinger, S.; Schmieg, B.; Jüngst, T.; Groll, J.; Meinel, L.; Schmidt, I.; Hartmann, H.; Schenke-Layland, K.; Brandt, N.; Selzer, M.; Zimmermann, S.; Koltay, P.; Southan, A.; Tovar, G. E. M.; Schmidt, S.; Weber, A.; Ahlfeld, T.; Gelinsky, M.; Scheibel, T.; Detsch, R.; Boccaccini, A. R.; Naolou, T.; Lee-Thedieck, C.; Willems, C.; Groth, T.; Allgeier, S.; Köhler, B.; Friedrich, T.; Briesen, H.; Buchholz, J.; Paulus, D.; von Gladiss, A.; Hubbuch, J.
2024. Biofabrication, 16 (1), Art.-Nr.: 015002. doi:10.1088/1758-5090/acfe3b

Fast HPLC-based affinity method to determine capsid titer and full/empty ratio of adeno-associated viral vectors
Heckel, J.; Martinez, A.; Elger, C.; Haindl, M.; Leiss, M.; Ruppert, R.; Williams, C.; Hubbuch, J.; Graf, T.
2023. Molecular Therapy - Methods & Clinical Development, 31, Art.-Nr.: 101148. doi:10.1016/j.omtm.2023.101148

The Effect of Gelatin Source on the Synthesis of Gelatin-Methacryloyl and the Production of Hydrogel Microparticles
Grijalva Garces, D.; Appoldt, L. J.; Egner, J.; Leister, N.; Hubbuch, J.
2023. Gels, 9 (12), Art.-Nr.: 927. doi:10.3390/gels9120927

Antibody sequence-based prediction of pH gradient elution in multimodal chromatography
Hess, R.; Faessler, J.; Yun, D.; Saleh, D.; Grosch, J.-H.; Schwab, T.; Hubbuch, J.
2023. Journal of Chromatography A, 1711, Art.-Nr.: 464437. doi:10.1016/j.chroma.2023.464437

Connected mechanistic process modeling to predict a commercial biopharmaceutical downstream process
Rischawy, F.; Briskot, T.; Hopf, N.; Saleh, D.; Wang, G.; Kluters, S.; Studts, J.; Hubbuch, J.
2023. Computers & Chemical Engineering, 176, Art.-Nr.: 108292. doi:10.1016/j.compchemeng.2023.108292

Monitoring of ultra- and diafiltration processes by Kalman-filtered Raman measurements
Rolinger, L.; Hubbuch, J.; Rüdt, M.
2023. Analytical and Bioanalytical Chemistry, 415 (5), 841–854. doi:10.1007/s00216-022-04477-7

Standardized method for mechanistic modeling of multimodal anion exchange chromatography in flow through operation
Hess, R.; Yun, D.; Saleh, D.; Briskot, T.; Grosch, J.-H.; Wang, G.; Schwab, T.; Hubbuch, J.
2023. Journal of Chromatography A, 1690, Art.-Nr.: 463789. doi:10.1016/j.chroma.2023.463789

Corrigendum: Kinetic studies and CFD-based reaction modeling for insights into the scalability of ADC conjugation reactions
Weggen, J. T.; Seidel, J.; Bean, R.; Wendeler, M.; Hubbuch, J.
2023. Frontiers in Bioengineering and Biotechnology, 11, Art.-Nr.: 1229416. doi:10.3389/fbioe.2023.1229416

Size-selective downstream processing of virus particles and non-enveloped virus-like particles
Hillebrandt, N.; Hubbuch, J.
2023. Frontiers in Bioengineering and Biotechnology, 11, Art.-Nr.: 1192050. doi:10.3389/fbioe.2023.1192050

An adaptive soft‐sensor for advanced real‐time monitoring of an antibody‐drug conjugation reaction
Schiemer, R.; Weggen, J. T.; Schmitt, K. M.; Hubbuch, J.
2023. Biotechnology and Bioengineering, 120 (7), 1914–1928. doi:10.1002/bit.28428

Analytics in Extrusion-Based Bioprinting: Standardized Methods Improving Quantification and Comparability of the Performance of Bioinks
Strauß, S.; Grijalva Garces, D.; Hubbuch, J.
2023. Polymers, 15 (8), Art.-Nr.: 1829. doi:10.3390/polym15081829

Kinetic studies and CFD-based reaction modeling for insights into the scalability of ADC conjugation reactions
Weggen, J. T.; Seidel, J.; Bean, R.; Wendeler, M.; Hubbuch, J.
2023. Frontiers in Bioengineering and Biotechnology, 11, Art.-Nr.: 1123842. doi:10.3389/fbioe.2023.1123842

Mechanical Properties of Protein-Based Hydrogels Derived from Binary Protein Mixtures—A Feasibility Study
Haas, S.; Hubbuch, J.
2023. Polymers, 15 (4), Art.-Nr.: 964. doi:10.3390/polym15040964

Bio-based material formulation for extrusion printing by dityrosine crosslinking of unmodified casein
Haas, S.; Götz, F.; Hubbuch, J.
2022. Bioprinting, 28, Art.Nr. e00245. doi:10.1016/j.bprint.2022.e00245

Automated and dynamic extrusion pressure adjustment based on real-time flow rate measurements for precise ink dispensing in 3D bioprinting
Wenger, L.; Strauß, S.; Hubbuch, J.
2022. Bioprinting, 28, Art.Nr. e00229. doi:10.1016/j.bprint.2022.e00229

Magnetic Resonance Imaging: Time-DependentWetting and Swelling Behavior of an Auxetic Hydrogel Based on Natural Polymers
Haas, S.; Schmieg, B.; Wendlich, P.; Guthausen, G.; Hubbuch, J.
2022. Polymers, 14 (22), Article no: 5023. doi:10.3390/polym14225023

Systematic evaluation of agarose- and agar-based bioinks for extrusion-based bioprinting of enzymatically active hydrogels
Wenger, L.; Radtke, C. P.; Gerisch, E.; Kollmann, M.; Niemeyer, C. M.; Rabe, K. S.; Hubbuch, J.
2022. Frontiers in Bioengineering and Biotechnology, 10, Art.-Nr.: 928878. doi:10.3389/fbioe.2022.928878

Integrated process model for the prediction of biopharmaceutical manufacturing chromatography and adjustment steps
Rischawy, F.; Briskot, T.; Schimek, A.; Wang, G.; Saleh, D.; Kluters, S.; Studts, J.; Hubbuch, J.
2022. Journal of Chromatography A, 1681, Art.-Nr.: 463421. doi:10.1016/j.chroma.2022.463421

A Novel MATLAB®-Algorithm-Based Video Analysis to Quantitatively Determine Solution Creeping in Intact Pharmaceutical Glass Vials
Molnar, D.; Röhm, M.; Wutz, J.; Rivec, I.; Michel, A.; Klotz, G.; Hubbuch, J.; Schindowski, K.; Presser, I.
2022. European Journal of Pharmaceutics and Biopharmaceutics, 178, 117–130. doi:10.1016/j.ejpb.2022.08.003

Structured Data Storage for Data-Driven Process Optimisation in Bioprinting
Schmieg, B.; Brandt, N.; Schnepp, V. J.; Radosevic, L.; Gretzinger, S.; Selzer, M.; Hubbuch, J.
2022. Applied Sciences, 12 (15), Artikel-Nr.: 7728. doi:10.3390/app12157728

Process development exploiting competitive adsorption-based displacement effects in monoclonal antibody aggregate removal—A new high-throughput screening procedure for membrane chromatography
Stein, D.; Thom, V.; Hubbuch, J.
2022. Biotechnology and Applied Biochemistry, 69 (4), 1663–1678. doi:10.1002/bab.2236

Steric exclusion chromatography of lentiviral vectors using hydrophilic cellulose membranes
Labisch, J. J.; Kassar, M.; Bollmann, F.; Valentic, A.; Hubbuch, J.; Pflanz, K.
2022. Journal of Chromatography A, 1674, Art.-Nr.: 463148. doi:10.1016/j.chroma.2022.463148

Streamlined process development procedure incorporating the selection of various stationary phase types established in a mAb aggregate reduction study with different mixed mode ligands
Stein, D.; Thom, V.; Hubbuch, J.
2022. Biotechnology Progress, 38 (2), Art. Nr.: e3230. doi:10.1002/btpr.3230

Rapid Analysis for Multicomponent High-Throughput Crystallization Screening: Combination of UV-Vis Spectroscopy and Chemometrics
Wegner, C. H.; Zimmermann, I.; Hubbuch, J.
2022. Crystal Growth and Design, 22 (2), 1054–1065. doi:10.1021/acs.cgd.1c00907

Calibration-free PAT: Locating selective crystallization or precipitation sweet spot in screenings with multi-way PARAFAC models
Wegner, C. H.; Hubbuch, J.
2022. Frontiers in Bioengineering and Biotechnology, 10, Art.-Nr. 1051129. doi:10.3389/fbioe.2022.1051129

A Novel Approach for the Manufacturing of Gelatin-Methacryloyl
Grijalva Garces, D.; Radtke, C. P.; Hubbuch, J.
2022. Polymers, 14 (24), Art.-Nr.: 5424. doi:10.3390/polym14245424

A multiscale modeling method for therapeutic antibodies in ion exchange chromatography
Saleh, D.; Hess, R.; Ahlers-Hesse, M.; Rischawy, F.; Wang, G.; Grosch, J.-H.; Schwab, T.; Kluters, S.; Studts, J.; Hubbuch, J.
2022. Biotechnology and Bioengineering, 120 (1), 125–138. doi:10.1002/bit.28258

Changing mechanical properties of photopolymerized, dityrosine-crosslinked protein-based hydrogels
Haas, S.; Körner, S.; Zintel, L.; Hubbuch, J.
2022. Frontiers in Bioengineering and Biotechnology, 10, Art.-Nr.: .1006438. doi:10.3389/fbioe.2022.1006438

Purification of a Hydrophobic Elastin-Like Protein Toward Scale-Suitable Production of Biomaterials
Haas, S.; Desombre, M.; Kirschhöfer, F.; Huber, M. C.; Schiller, S. M.; Hubbuch, J.
2022. Frontiers in Bioengineering and Biotechnology, 10, Art.-Nr.: 878838. doi:10.3389/fbioe.2022.878838

Effects of Different Lengths of a Nucleic Acid Binding Region and Bound Nucleic Acids on the Phase Behavior and Purification Process of HBcAg Virus-Like Particles
Valentic, A.; Müller, J.; Hubbuch, J.
2022. Frontiers in Bioengineering and Biotechnology, 10, Art.-Nr.: 929243. doi:10.3389/fbioe.2022.929243

Virtual Reality as Tool for Bioprinting Quality Inspection: A Proof of Principle
Gretzinger, S.; Schmieg, B.; Guthausen, G.; Hubbuch, J.
2022. Frontiers in Bioengineering and Biotechnology, 10, Art.-Nr.: 895842. doi:10.3389/fbioe.2022.895842

Investigation of Lysozyme Diffusion in Agarose Hydrogels Employing a Microfluidics-Based UV Imaging Approach
Wenger, L.; Hubbuch, J.
2022. Frontiers in Bioengineering and Biotechnology, 10, Art.-Nr.: 849271. doi:10.3389/fbioe.2022.849271

Evaluation of the Reproducibility and Robustness of Extrusion-Based Bioprinting Processes Applying a Flow Sensor
Strauß, S.; Schroth, B.; Hubbuch, J.
2022. Frontiers in Bioengineering and Biotechnology, 10, Artkl.Nr.: 831350. doi:10.3389/fbioe.2022.831350

Magnetic resonance imaging as a tool for quality control in extrusion-based bioprinting
Schmieg, B.; Gretzinger, S.; Schuhmann, S.; Guthausen, G.; Hubbuch, J.
2022. Biotechnology Journal, 17 (5), Art.-Nr.: 2100336. doi:10.1002/biot.202100336

Process monitoring framework for cross-flow diafiltration-based virus-like particle disassembly: Tracing product properties and filtration performance
Hillebrandt, N.; Vormittag, P.; Dietrich, A.; Hubbuch, J.
2022. Biotechnology and Bioengineering, 119 (6), 1522–1538. doi:10.1002/bit.28063

Modeling the Gibbs–Donnan effect during ultrafiltration and diafiltration processes using the Poisson–Boltzmann theory in combination with a basic Stern model
Briskot, T.; Hillebrandt, N.; Kluters, S.; Wang, G.; Studts, J.; Hahn, T.; Huuk, T.; Hubbuch, J.
2022. Journal of Membrane Science, 648, Art.-Nr.: 120333. doi:10.1016/j.memsci.2022.120333

Synthesis of Spherical Nanoparticle Hybrids via Aerosol Thiol-Ene Photopolymerization and Their Bioconjugation
Suvarli, N.; Frentzel, M.; Hubbuch, J.; Perner-Nochta, I.; Wörner, M.
2022. Nanomaterials, 12 (3), Art.-Nr.: 577. doi:10.3390/nano12030577

Immobilization of β-Galactosidase by Encapsulation of Enzyme-Conjugated Polymer Nanoparticles Inside Hydrogel Microparticles
Suvarli, N.; Wenger, L.; Serra, C.; Perner-Nochta, I.; Hubbuch, J.; Wörner, M.
2022. Frontiers in Bioengineering and Biotechnology, 9, Art.-Nr.: 818053. doi:10.3389/fbioe.2021.818053

Raman spectroscopy as a process analytical technology to investigate biopharmaceutical freeze concentration processes
Weber, D.; Hubbuch, J.
2021. Biotechnology & bioengineering, 118 (12), 4708–4719. doi:10.1002/bit.27936

Impact of freeze–thaw processes on monoclonal antibody platform process development
Weber, D.; Sittig, C.; Hubbuch, J.
2021. Biotechnology & bioengineering, 118 (10), 3914–3925. doi:10.1002/bit.27867

Piezoelectric Silicon Micropump for Drug Delivery Applications
Bußmann, A.; Leistner, H.; Zhou, D.; Wackerle, M.; Congar, Y.; Richter, M.; Hubbuch, J.
2021. Applied Sciences (Switzerland), 11 (17), Art.-Nr.: 8008. doi:10.3390/app11178008

Image analysis as PAT-Tool for use in extrusion-based bioprinting
Strauß, S.; Meutelet, R.; Radosevic, L.; Gretzinger, S.; Hubbuch, J.
2021. Bioprinting, 21, Art. Nr.: e00112. doi:10.1016/j.bprint.2020.e00112

A multisensor approach for improved protein A load phase monitoring by conductivity-based background subtraction of UV spectra
Rolinger, L.; Rüdt, M.; Hubbuch, J.
2021. Biotechnology & bioengineering, 118 (2), 905–917. doi:10.1002/bit.27616

Cross-scale quality assessment of a mechanistic cation exchange chromatography model
Saleh, D.; Wang, G.; Mueller, B.; Rischawy, F.; Kluters, S.; Studts, J.; Hubbuch, J.
2021. Biotechnology progress, 37 (1), Art.Nr. e3081. doi:10.1002/btpr.3081

Thiol-functional polymer nanoparticles via aerosol photopolymerization
Suvarli, N.; Perner-Nochta, I.; Hubbuch, J.; Wörner, M.
2021. Polymers, 13 (24), 4363. doi:10.3390/polym13244363

Microfluidic cell transport with piezoelectric micro diaphragm pumps
Bußmann, A.; Thalhofer, T.; Hoffmann, S.; Daum, L.; Surendran, N.; Hayden, O.; Hubbuch, J.; Richter, M.
2021. Micromachines, 12 (12), 1459. doi:10.3390/mi12121459

Comparison of UV- and Raman-based monitoring of the Protein A load phase and evaluation of data fusion by PLS models and CNNs
Rolinger, L.; Rüdt, M.; Hubbuch, J.
2021. Biotechnology and Bioengineering, 118 (11), 4255–4268. doi:10.1002/bit.27894

Exploration of fiber-based cation exchange adsorbents for the removal of monoclonal antibody aggregates
Winderl, J.; Neumann, E.; Hubbuch, J.
2021. Journal of Chromatography A, 1654, Art.-Nr.: 462451. doi:10.1016/j.chroma.2021.462451

High throughput screening of fiber-based adsorbents for material and process development
Winderl, J.; Bürkle, S.; Hubbuch, J.
2021. Journal of Chromatography A, 1653, Art.-Nr.: 462387. doi:10.1016/j.chroma.2021.462387

Analysis of complex protein elution behavior in preparative ion exchange processes using a colloidal particle adsorption model
Briskot, T.; Hahn, T.; Huuk, T.; Wang, G.; Kluters, S.; Studts, J.; Wittkopp, F.; Winderl, J.; Schwan, P.; Hagemann, I.; Kaiser, K.; Trapp, A.; Stamm, S. M.; Koehn, J.; Malmquist, G.; Hubbuch, J.
2021. Journal of Chromatography A, 1654, Art.-Nr.: 462439. doi:10.1016/j.chroma.2021.462439

In silico process characterization for biopharmaceutical development following the quality by design concept
Saleh, D.; Wang, G.; Rischawy, F.; Kluters, S.; Studts, J.; Hubbuch, J.
2021. Biotechnology progress, 37 (6), e3196. doi:10.1002/btpr.3196

Protein adsorption on ion exchange adsorbers: A comparison of a stoichiometric and non-stoichiometric modeling approach
Briskot, T.; Hahn, T.; Huuk, T.; Hubbuch, J.
2021. Journal of Chromatography A, 1653, Art.-Nr.: 462397. doi:10.1016/j.chroma.2021.462397

Process development for cross-flow diafiltration-based VLP disassembly: A novel high-throughput screening approach
Hillebrandt, N.; Vormittag, P.; Dietrich, A.; Wegner, C. H.; Hubbuch, J.
2021. Biotechnology & bioengineering, 118 (10), 3926–3940. doi:10.1002/bit.27868

Application of ultraviolet, visible, and infrared light imaging in protein-based biopharmaceutical formulation characterization and development studies
Klijn, M. E.; Hubbuch, J.
2021. European Journal of Pharmaceutics and Biopharmaceutics, 165, 319–336. doi:10.1016/j.ejpb.2021.05.013

Modeling the impact of amino acid substitution in a monoclonal antibody on cation exchange chromatography
Saleh, D.; Hess, R.; Ahlers-Hesse, M.; Beckert, N.; Schönberger, M.; Rischawy, F.; Wang, G.; Bauer, J.; Blech, M.; Kluters, S.; Studts, J.; Hubbuch, J.
2021. Biotechnology and Bioengineering, 118 (8), 2923–2933. doi:10.1002/bit.27798

Temperature Based Process Characterization of Pharmaceutical Freeze-Thaw Operations
Weber, D.; Hubbuch, J.
2021. Frontiers in Bioengineering and Biotechnology, 9, Art.-Nr.: 617770. doi:10.3389/fbioe.2021.617770

High throughput screening setup of a scale-down device for membrane chromatography-aggregate removal of monoclonal antibodies
Stein, D.; Thom, V.; Hubbuch, J.
2020. Biotechnology progress, 36 (6), Art.Nr. e3055. doi:10.1002/btpr.3055

On the analysis of chromatographic biopharmaceutical data by curve resolution techniques in the framework of the area of feasible solutions
Sawall, M.; Rüdt, M.; Hubbuch, J.; Neymeyr, K.
2020. Journal of Chromatography A, 1627, 461420. doi:10.1016/j.chroma.2020.461420

Straightforward method for calibration of mechanistic cation exchange chromatography models for industrial applications
Saleh, D.; Wang, G.; Müller, B.; Rischawy, F.; Kluters, S.; Studts, J.; Hubbuch, J.
2020. Biotechnology progress, 36 (4), Art.Nr. e2984. doi:10.1002/btpr.2984

Modeling of hydrophobic interaction chromatography for the separation of antibody-drug conjugates and its application towards quality by design
Andris, S.; Hubbuch, J.
2020. Journal of biotechnology, 317, 48–58. doi:10.1016/j.jbiotec.2020.04.018

Influence of image analysis strategy, cooling rate, and sample volume on apparent protein cloud-point temperature determination
Klijn, M. E.; Hubbuch, J.
2020. Bioprocess and biosystems engineering, 44 (3), 525–536. doi:10.1007/s00449-020-02465-8

Optimization of a Soft Ensemble Vote Classifier for the Prediction of Chimeric Virus-Like Particle Solubility and Other Biophysical Properties
Vormittag, P.; Klamp, T.; Hubbuch, J.
2020. Frontiers in Bioengineering and Biotechnology, 8, Art.-Nr.: 881. doi:10.3389/fbioe.2020.00881

3D-Printable and Enzymatically Active Composite Materials Based on Hydrogel-Filled High Internal Phase Emulsions
Wenger, L.; Radtke, C. P.; Göpper, J.; Wörner, M.; Hubbuch, J.
2020. Frontiers in Bioengineering and Biotechnology, 8, Art. Nr.: 713. doi:10.3389/fbioe.2020.00713

Integrated Process for Capture and Purification of Virus-Like Particles: Enhancing Process Performance by Cross-Flow Filtration
Hillebrandt, N.; Vormittag, P.; Bluthardt, N.; Dietrich, A.; Hubbuch, J.
2020. Frontiers in Bioengineering and Biotechnology, 8, Aericle No.489. doi:10.3389/fbioe.2020.00489

Ensembles of Hydrophobicity Scales as Potent Classifiers for Chimeric Virus-Like Particle Solubility – An Amino Acid Sequence-Based Machine Learning Approach
Vormittag, P.; Klamp, T.; Hubbuch, J.
2020. Frontiers in Bioengineering and Biotechnology, 8, Art. Nr.: 395. doi:10.3389/fbioe.2020.00395

Investigation of the reversibility of freeze/thaw stress-induced protein instability using heat cycling as a function of different cryoprotectants
Wöll, A. K.; Hubbuch, J.
2020. Bioprocess and biosystems engineering, 43, 1309–1327. doi:10.1007/s00449-020-02327-3

A critical review of recent trends, and a future perspective of optical spectroscopy as PAT in biopharmaceutical downstream processing
Rolinger, L.; Rüdt, M.; Hubbuch, J.
2020. Analytical and bioanalytical chemistry, 412 (9), 2047–2064. doi:10.1007/s00216-020-02407-z

Adsorption of colloidal proteins in ion-exchange chromatography under consideration of charge regulation
Briskot, T.; Hahn, T.; Huuk, T.; Hubbuch, J.
2020. Journal of chromatography / A, 1611, Art.Nr. 460608. doi:10.1016/j.chroma.2019.460608

Multi-attribute PAT for UF/DF of Proteins—Monitoring Concentration, particle sizes, and Buffer Exchange
Rolinger, L.; Rüdt, M.; Diehm, J.; Chow-Hubbertz, J.; Heitmann, M.; Schleper, S.; Hubbuch, J.
2020. Analytical and bioanalytical chemistry, 412 (9), 2123–2136. doi:10.1007/s00216-019-02318-8

Apparent protein cloud point temperature determination using a low volume high-throughput cryogenic device in combination with automated imaging
Klijn, M. E.; Wöll, A. K.; Hubbuch, J.
2020. Bioprocess and biosystems engineering, 43, 439–456. doi:10.1007/s00449-019-02239-x

A phase diagram-based toolbox to assess the impact of freeze/thaw ramps on the phase behavior of proteins
Wöll, A. K.; Desombre, M.; Enghauser, L.; Hubbuch, J.
2020. Bioprocess and biosystems engineering, 43, 179–192. doi:10.1007/s00449-019-02215-5

Time-Dependent Multi-Light-Source Image Classification Combined With Automated Multidimensional Protein Phase Diagram Construction for Protein Phase Behavior Analysis
Klijn, M. E.; Hubbuch, J.
2020. Journal of pharmaceutical sciences, 109 (1), 331–339. doi:10.1016/j.xphs.2019.07.017

Kinetic reaction modeling for antibody-drug conjugate process development
Andris, S.; Seidel, J.; Hubbuch, J.
2019. Journal of biotechnology, 306, 71–80. doi:10.1016/j.jbiotec.2019.09.013

Machine-assisted cultivation and analysis of biofilms
Hansen, S. H.; Kabbeck, T.; Radtke, C. P.; Krause, S.; Krolitzki, E.; Peschke, T.; Gasmi, J.; Rabe, K. S.; Wagner, M.; Horn, H.; Hubbuch, J.; Gescher, J.; Niemeyer, C. M.
2019. Scientific reports, 9 (1), Article: 8933. doi:10.1038/s41598-019-45414-6

Water on hydrophobic surfaces: mechanistic modeling of polyethylene glycol-induced protein precipitation
Großhans, S.; Wang, G.; Hubbuch, J.
2019. Bioprocess and biosystems engineering, 42 (4), 513–520. doi:10.1007/s00449-018-2054-5

Prediction uncertainty assessment of chromatography models using Bayesian inference
Briskot, T.; Stückler, F.; Wittkopp, F.; Williams, C.; Yang, J.; Konrad, S.; Doninger, K.; Griesbach, J.; Bennecke, M.; Hepbildikler, S.; Hubbuch, J.
2019. Journal of chromatography / A, 1587, 101–110. doi:10.1016/j.chroma.2018.11.076

Modifying an ÄKTApurifier System for the Automated Acquisition of Samples for Kinetic Modeling of Batch Reactions
Sanden, A.; Haas, S.; Hubbuch, J.
2019. SLAS technology, 25 (2), 106–110. doi:10.1177/2472630319891976

Fourier-transform infrared spectroscopy as a process analytical technology for near real time in-line estimation of the degree of PEGylation in chromatography
Sanden, A.; Suhm, S.; Rüdt, M.; Hubbuch, J.
2019. Journal of chromatography / A, 1608, Article: 460410. doi:10.1016/j.chroma.2019.460410

3D‐Printed Phenacrylate Decarboxylase Flow Reactors for the Chemoenzymatic Synthesis of 4‐Hydroxystilbene
Peng, M.; Mittmann, E.; Wenger, L.; Hubbuch, J.; Engqvist, M. K. M.; Niemeyer, C. M.; Rabe, K. S.
2019. Chemistry - a European journal, 25 (70), 15998–16001. doi:10.1002/chem.201904206

Preparative Protein Crystallization
Hubbuch, J.; Kind, M.; Nirschl, H.
2019. Chemical engineering & technology, 42 (11), 2275–2281. doi:10.1002/ceat.201800627

Redesigning food protein formulations with empirical phase diagrams: A case study on glycerol-poor and glycerol-free formulations
Klijn, M. E.; Hubbuch, J.
2019. Food research international, 125, Art.-Nr.: 108609. doi:10.1016/j.foodres.2019.108609

Process monitoring of virus-like particle reassembly by diafiltration with UV/Vis spectroscopy and light scattering
Rüdt, M.; Vormittag, P.; Hillebrandt, N.; Hubbuch, J.
2019. Biotechnology & bioengineering, 116 (6), 1366–1379. doi:10.1002/bit.26935

High-throughput computational pipeline for 3-D structure preparation and in silico protein surface property screening: A case study on HBcAg dimer structures
Klijn, M. E.; Vormittag, P.; Bluthardt, N.; Hubbuch, J.
2019. International journal of pharmaceutics, 563, 337–346. doi:10.1016/j.ijpharm.2019.03.057

Precipitation of complex antibody solutions: influence of contaminant composition and cell culture medium on the precipitation behavior
Großhans, S.; Suhm, S.; Hubbuch, J.
2019. Bioprocess and biosystems engineering, 42 (6), 1039–1051. doi:10.1007/s00449-019-02103-y

Correlating multidimensional short-term empirical protein properties to long-term protein physical stability data via empirical phase diagrams
Klijn, M. E.; Hubbuch, J.
2019. International journal of pharmaceutics, 560, 166–174. doi:10.1016/j.ijpharm.2019.02.006

Automated image processing as an analytical tool in cell cryopreservation for bioprocess development
Gretzinger, S.; Limbrunner, S.; Hubbuch, J.
2019. Bioprocess and biosystems engineering, 42 (5), 665–675. doi:10.1007/s00449-019-02071-3

Factorization of preparative protein chromatograms with hard-constraint multivariate curve resolution and second-derivative pretreatment
Rüdt, M.; Andris, S.; Schiemer, R.; Hubbuch, J.
2019. Journal of chromatography, 1585, 152–160. doi:10.1016/j.chroma.2018.11.065

Analysis of phase behavior and morphology during freeze-thaw applications of lysozyme
Wöll, A. K.; Schütz, J.; Zabel, J.; Hubbuch, J.
2019. International journal of pharmaceutics, 555, 153–164. doi:10.1016/j.ijpharm.2018.11.047

Impact of Polymer Bioconjugation on Protein Stability and Activity Investigated with Discrete Conjugates: Alternatives to PEGylation
Morgenstern, J.; Gil Alvaradejo, G.; Bluthardt, N.; Beloqui, A.; Delaittre, G.; Hubbuch, J.
2018. Biomacromolecules, 19 (11), 4250–4262. doi:10.1021/acs.biomac.8b01020

Application of Empirical Phase Diagrams for Multidimensional Data Visualization of High-Throughput Microbatch Crystallization Experiments
Klijn, M. E.; Hubbuch, J.
2018. Journal of pharmaceutical sciences, 107 (8), 2063–2069. doi:10.1016/j.xphs.2018.04.018

Multi-step high-throughput conjugation platform for the development of antibody-drug conjugates
Andris, S.; Wendeler, M.; Wang, X.; Hubbuch, J.
2018. Journal of biotechnology, 278, 48–55. doi:10.1016/j.jbiotec.2018.05.004

Packing characteristics of winged shaped polymer fiber supports for preparative chromatography
Winderl, J.; Spies, T.; Hubbuch, J.
2018. Journal of chromatography / A, 1553, 67–80. doi:10.1016/j.chroma.2018.04.020

On-Demand Production of Flow-Reactor Cartridges by 3D Printing of Thermostable Enzymes
Maier, M.; Radtke, C. P.; Hubbuch, J.; Niemeyer, C. M.; Rabe, K. S.
2018. Angewandte Chemie / International edition, 57 (19), 5539–5543. doi:10.1002/anie.201711072

In-line Fourier-transform infrared spectroscopy as a versatile process analytical technology for preparative protein chromatography
Großhans, S.; Rüdt, M.; Sanden, A.; Brestrich, N.; Morgenstern, J.; Heissler, S.; Hubbuch, J.
2018. Journal of chromatography / A, 1547, 37–44. doi:10.1016/j.chroma.2018.03.005

The Biomaker : An entry-level bioprinting device for biotechnological applications
Radtke, C. P.; Hillebrandt, N.; Hubbuch, J.
2018. Journal of chemical technology & biotechnology, 93 (3), 792–799. doi:10.1002/jctb.5429

Cell Separation in Aqueous Two-Phase Systems − Influence of Polymer Molecular Weight and Tie-Line Length on the Resolution of Five Model Cell Lines
Zimmermann, S.; Gretzinger, S.; Zimmermann, P. K.; Bogsnes, A.; Hansson, M.; Hubbuch, J.
2018. Biotechnology journal, 13 (2), Art. Nr.: 1700250. doi:10.1002/biot.201700250

Selective protein quantification for preparative chromatography using variable pathlength UV/Vis spectroscopy and partial least squares regression
Brestrich, N.; Rüdt, M.; Büchler, D.; Hubbuch, J.
2018. Chemical engineering science, 176, 157–164. doi:10.1016/j.ces.2017.10.030

An integrated precipitation and ion-exchange chromatography process for antibody manufacturing : Process development strategy and continuous chromatography exploration
Großhans, S.; Wang, G.; Fischer, C.; Hubbuch, J.
2018. Journal of chromatography / A, 1533, 66–76. doi:10.1016/j.chroma.2017.12.013

Monitoring of antibody-drug conjugation reactions with UV/Vis spectroscopy
Andris, S.; Rüdt, M.; Rogalla, J.; Wendeler, M.; Hubbuch, J.
2018. Journal of biotechnology, 288, 15–22. doi:10.1016/j.jbiotec.2018.10.003

3D bioprinting – Flow cytometry as analytical strategy for 3D cell structures
Gretzinger, S.; Beckert, N.; Gleadall, A.; Lee-Thedieck, C.; Hubbuch, J.
2018. Bioprinting, 11, e00023. doi:10.1016/j.BPRINT.2018.e00023

Machine-assisted cultivation and analysis of biofilms
Hansen, S. H.; Kabbeck, T.; Radtke, C. P.; Krause, S.; Krolitzki, E.; Peschke, T.; Gasmi, J.; Rabe, K. S.; Wagner, M.; Horn, H.; Hubbuch, J.; Gescher, J.; Niemeyer, C. M.
2018. Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie / VAAM (2018), Wolfsburg, Deutschland, 15.–18. April 2018

Real-time monitoring and control of the load phase of a protein A capture step
Rüdt, M.; Brestrich, N.; Rolinger, L.; Hubbuch, J.
2017. Biotechnology & bioengineering, 114 (2), 368–373. doi:10.1002/bit.26078

Mid-UV Protein Absorption Spectra and Partial Least Squares Regression as Screening and PAT Tool
Hansen, S.; Brestrich, N.; Staby, A.; Hubbuch, J.
2017. Preparative Chromatography for Separation of Proteins. Ed.: Arne Staby, 501–536, John Wiley and Sons. doi:10.1002/9781119031116.ch17

High-Throughput Column Chromatography Performed on Liquid Handling Stations – Process Characterization and Error Analysis
Diederich, P.; Hubbuch, J.
2017. Preparative Chromatography for Separation of Proteins. Ed.: A. Staby, 293–332, John Wiley and Sons. doi:10.1002/9781119031116.ch10

Antibody fingerprints in lyme disease deciphered with high density peptide arrays
Weber, L. K.; Isse, A.; Rentschler, S.; Kneusel, R. E.; Palermo, A.; Hubbuch, J.; Nesterov-Mueller, A.; Breitling, F.; Loeffler, F. F.
2017. Engineering in life sciences, 17 (10), 1078–1087. doi:10.1002/elsc.201700062

Model-Based Investigation on the Mass Transfer and Adsorption Mechanisms of Mono-Pegylated Lysozyme in Ion-Exchange Chromatography
Morgenstern, J.; Wang, G.; Baumann, P.; Hubbuch, J. J.
2017. Biotechnology journal, 12 (9), Art.Nr.: 1700255. doi:10.1002/biot.201700255

Identification of a Tetanus Toxin Specific Epitope in Single Amino Acid Resolution
Palermo, A.; Weber, L. K.; Rentschler, S.; Isse, A.; Sedlmayr, M.; Herbster, K.; List, V.; Hubbuch, J.; Löffler, F. F.; Nesterov-Müller, A.; Breitling, F.
2017. Biotechnology journal, 12 (10), Art.Nr. 1700197/1–8. doi:10.1002/biot.201700197

Prediction and characterization of the stability enhancing effect of the Cherry-Tag™ in highly concentrated protein solutions by complex rheological measurements and MD simulations
Baumann, P.; Schermeyer, M.-T.; Burghardt, H.; Dürr, C.; Gärtner, J.; Hubbuch, J.
2017. International journal of pharmaceutics, 531 (1), 360–371. doi:10.1016/j.ijpharm.2017.08.068

Root cause investigation of deviations in protein chromatography based on mechanistic models and artificial neural networks
Wang, G.; Briskot, T.; Hahn, T.; Baumann, P.; Hubbuch, J.
2017. Journal of chromatography / A, 1515, 146–153. doi:10.1016/j.chroma.2017.07.089

Influence of the production system on the surface properties of influenza A virus particles
Hämmerling, F.; Pieler, M. M.; Hennig, R.; Serve, A.; Rapp, E.; Wolff, M. W.; Reichl, U.; Hubbuch, J.
2017. Engineering in life sciences, 17 (10), 1071–1077. doi:10.1002/elsc.201700058

Feasibility of using continuous chromatography in downstream processing : Comparison of costs and product quality for a hybrid process vs. a conventional batch process
Ötes, O.; Flato, H.; Winderl, J.; Hubbuch, J.; Capito, F.
2017. Journal of biotechnology, 259, 213–220. doi:10.1016/j.jbiotec.2017.07.001

Characterization of highly concentrated antibody solution : A toolbox for the description of protein long-term solution stability
Schermeyer, M. T.; Wöll, A. K.; Kokke, B.; Eppink, M. H. M.; Hubbuch, J. J.
2017. mAbs, 9 (7), 1169 – 1185. doi:10.1080/19420862.2017.1338222

Orientation of monoclonal antibodies in ion-exchange chromatography: A predictive quantitative structure-activity relationship modeling approach
Kittelmann, J.; Lang, K. M. H.; Ottens, M.; Hubbuch, J.
2017. Journal of chromatography / A, 1510, 33–39. doi:10.1016/j.chroma.2017.06.047

Predictive approach for protein aggregation: Correlation of protein surface characteristics and conformational flexibility to protein aggregation propensity
Galm, L.; Amrhein, S.; Hubbuch, J.
2017. Biotechnology & bioengineering, 114 (6), 1170–1183. doi:10.1002/bit.25949

Modeling of complex antibody elution behavior under high protein load densities in ion exchange chromatography using an asymmetric activity coefficient
Huuk, T. C.; Hahn, T.; Doninger, K.; Griesbach, J.; Hepbildikler, S.; Hubbuch, J.
2017. Biotechnology journal, 12 (3), 1600336/1–8. doi:10.1002/biot.201600336

High-throughput downstream process development for cell-based products using aqueous two-phase systems (ATPS) – A case study
Zimmermann, S.; Scheeder, C.; Zimmermann, P. K.; Bogsnes, A.; Hansson, M.; Staby, A.; Hubbuch, J.
2017. Biotechnology journal, 12 (2), 1600587. doi:10.1002/biot.201600587

Effect of PEG molecular weight and PEGylation degree on the physical stability of PEGylated lysozyme
Morgenstern, J.; Baumann, P.; Brunner, C.; Hubbuch, J.
2017. International journal of pharmaceutics, 519 (1-2), 408–417. doi:10.1016/j.ijpharm.2017.01.040

Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays
Weber, L. K.; Palermo, A.; Kügler, J.; Armant, O.; Isse, A.; Rentschler, S.; Jaenisch, T.; Hubbuch, J.; Dübel, S.; Nesterov-Mueller, A.; Breitling, F.; Loeffler, F. F.
2017. Journal of immunological methods, 443, 45–54. doi:10.1016/j.jim.2017.01.012

Influence of structure properties on protein–protein interactions—QSAR modeling of changes in diffusion coefficients
Bauer, K. C.; Hämmerling, F.; Kittelmann, J.; Dürr, C.; Görlich, F.; Hubbuch, J.
2017. Biotechnology & bioengineering, 114 (4), 821–831. doi:10.1002/bit.26210

Estimation of adsorption isotherm and mass transfer parameters in protein chromatography using artificial neural networks
Wang, G.; Briskot, T.; Hahn, T.; Baumann, P.; Hubbuch, J.
2017. Journal of chromatography / A, 1487, 211–217. doi:10.1016/j.chroma.2017.01.068

Strategy for assessment of the colloidal and biological stability of H1N1 influenza A viruses
Hämmerling, F.; Lorenz-Cristea, O.; Baumann, P.; Hubbuch, J.
2017. International journal of pharmaceutics, 517 (1-2), 80–87. doi:10.1016/j.ijpharm.2016.11.058

An orientation sensitive approach in biomolecule interaction quantitative structure–activity relationship modeling and its application in ion-exchange chromatography
Kittelmann, J.; Lang, K. M. H.; Ottens, M.; Hubbuch, J.
2017. Journal of chromatography / A, 1482, 48–56. doi:10.1016/j.chroma.2016.12.065

Investigation and prediction of protein precipitation by polyethylene glycol using quantitative structure–activity relationship models
Hämmerling, F.; Ladd Effio, C.; Andris, S.; Kittelmann, J.; Hubbuch, J.
2017. Journal of biotechnology, 241, 87–97. doi:10.1016/j.jbiotec.2016.11.014

Impact of additives on the formation of protein aggregates and viscosity in concentrated protein solutions
Bauer, K. C.; Suhm, S.; Wöll, A. K.; Hubbuch, J.
2017. International journal of pharmaceutics, 516 (1-2), 82–90. doi:10.1016/j.ijpharm.2016.11.009

Downstream process development strategies for effective bioprocesses : Trends, progress, and combinatorial approaches
Baumann, P.; Hubbuch, J.
2017. Engineering in life sciences, 17 (11), 1142–1158. doi:10.1002/elsc.201600033

Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts
Buyel, J. F.; Hubbuch, J.; Fischer, R.
2016. Journal of visualized experiments, (114), Art. Nr.: e54343. doi:10.3791/54343

Advances in downstream processing of biologics - Spectroscopy: An emerging process analytical technology
Rüdt, M.; Briskot, T.; Hubbuch, J.
2016. Journal of chromatography / A. doi:10.1016/j.chroma.2016.11.010

Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles
Effio, C. L.; Hahn, T.; Seiler, J.; Oelmeier, S. A.; Asen, I.; Silberer, C.; Villain, L.; Hubbuch, J.
2016. Journal of chromatography, 1429, 142–154. doi:10.1016/j.chroma.2015.12.006

High-throughput process development of an alternative platform for the production of virus-like particles in Escherichia coli
Effio, C. L.; Baumann, P.; Weigel, C.; Vormittag, P.; Middelberg, A.; Hubbuch, J.
2016. Journal of biotechnology, 219, 7–19. doi:10.1016/j.jbiotec.2015.12.018

A mechanistic model of ion-exchange chromatography on polymer fiber stationary phases
Winderl, J.; Hahn, T.; Hubbuch, J.
2016. Journal of chromatography / A, 1475, 18–30. doi:10.1016/j.chroma.2016.10.057

Deconvolution of high-throughput multicomponent isotherms using multivariate data analysis of protein spectra
Baumann, P.; Huuk, T.; Hahn, T.; Osberghaus, A.; Hubbuch, J.
2016. Engineering in life sciences, 16 (2), 194–201. doi:10.1002/elsc.201400243

Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification
Hahn, T.; Huuk, T.; Osberghaus, A.; Doninger, K.; Nath, S.; Hepbildikler, S.; Heuveline, V.; Hubbuch, J.
2016. Engineering in life sciences, 16 (2), 107–113. doi:10.1002/elsc.201400248

UV absorption-based inverse modeling of protein chromatography
Hahn, T.; Baumann, P.; Huuk, T.; Heuveline, V.; Hubbuch, J.
2016. Engineering in life sciences, 16 (2), 99–106. doi:10.1002/elsc.201400247

High-throughput characterization of virus-like particles by interlaced size-exclusion chromatography
Effio, C. L.; Oelmeier, S. A.; Hubbuch, J.
2016. Vaccine, 34 (10), 1259–1267. doi:10.1016/j.vaccine.2016.01.035

Implementation of an analytical microfluidic device for the quantification of protein concentrations in high-throughput format
Radtke, C. P.; Schermeyer, M.-T.; Zhai, Y. C.; Goepper, J.; Hubbuch, J.
2016. Engineering in life sciences / Special Issue: Molecular Interaction Engineering, 16 (6), 515–524. doi:10.1002/elsc.201500185

Photoinitiated miniemulsion polymerization in microfluidic chips on automated liquid handling stations: Proof of concept
Radtke, C. P.; Delbe, M.; Woerner, M.; Hubbuch, J.
2016. Engineering in life sciences / Special Issue: Molecular Interaction Engineering, 16 (6), 505–514. doi:10.1002/elsc.201500186

Surface tension determination by means of liquid handling stations
Amrhein, S.; Suhm, S.; Hubbuch, J.
2016. Engineering in life sciences / Special Issue: Molecular Interaction Engineering, 16 (6), 532–537. doi:10.1002/elsc.201500179

Water on hydrophobic surfaces: Mechanistic modeling of hydrophobic interaction chromatography
Wang, G.; Hahn, T.; Hubbuch, J.
2016. Journal of chromatography / A, 1465, 71–78. doi:10.1016/j.chroma.2016.07.085

Quantification of PEGylated proteases with varying degree of conjugation in mixtures: An analytical protocol combining protein precipitation and capillary gel electrophoresis
Morgenstern, J.; Busch, M.; Baumann, P.; Hubbuch, J.
2016. Journal of chromatography / A, 1462, 153–164. doi:10.1016/j.chroma.2016.07.078

High-throughput downstream process development for cell-based products using aqueous two-phase systems
Zimmermann, S.; Gretzinger, S.; Schwab, M.-L.; Scheeder, C.; Zimmermann, P. K.; Oelmeier, S. A.; Gottwald, E.; Bogsnes, A.; Hansson, M.; Staby, A.; Hubbuch, J.
2016. Journal of chromatography / A, 1464, 1–11. doi:10.1016/j.chroma.2016.08.025

Application of spectral deconvolution and inverse mechanistic modelling as a tool for root cause investigation in protein chromatography
Brestrich, N.; Hahn, T.; Hubbuch, J.
2016. Journal of chromatography / A, 1437, 158–167. doi:10.1016/j.chroma.2016.02.011

Concentration-dependent changes in apparent diffusion coefficients as indicator for colloidal stability of protein solutions
Bauer, K. C.; Göbel, M.; Schwab, M.-L.; Schermeyer, M.-T.; Hubbuch, J.
2016. International journal of pharmaceutics, 511 (1), 276–287. doi:10.1016/j.ijpharm.2016.07.007

A versatile noninvasive method for adsorber quantification in batch and column chromatography based on the ionic capacity
Huuk, T. C.; Briskot, T.; Hahn, T.; Hubbuch, J. J.
2016. Biotechnology Progress, 32 (3), 666–677. doi:10.1002/btpr.2228

Automated microfluidic system with optical setup for the inverstigation of peptide-antibody interactions in an array format
Weber, L. K.; Fischer, A.; Schorb, T.; Soehindrijo, M.; Förtsch, T. C.; Bojnicic-Kninski, C. von; Althuon, D.; Leffler, F. F.; Breitling, F.; Hubbuch, J.; Nesterov-Müller, A.
2016. Microsystem Technology in Germany, 50–51

High-throughput cell quantification assays for use in cell purification development - enabling technologies for cell production
Zimmermann, S.; Gretzinger, S.; Scheeder, C.; Schwab, M.-L.; Oelmeier, S. A.; Osberghaus, A.; Gottwald, E.; Hubbuch, J.
2016. Biotechnology journal, 11 (5), 676–686. doi:10.1002/biot.201500577

The influence of mixed salts on the capacity of HIC adsorbers: A predictive correlation to the surface tension and the aggregation temperature
Baumgartner, K.; Amrhein, S.; Oelmeier, S. A.; Hubbuch, J.
2016. Biotechnology progress, 32 (2), 346–354. doi:10.1002/btpr.2166

Prediction of salt effects on protein phase behavior by HIC retention and thermal stability
Baumgartner, K.; Großhans, S.; Schütz, J.; Suhm, S.; Hubbuch, J. J.
2016. Journal of pharmaceutical and biomedical analysis, 128 (Sept.), 216–225. doi:10.1016/j.jpba.2016.04.040

Impact of polymer surface characteristics on the microrheological measurement quality of protein solutions : A tracer particle screening
Bauer, K. C.; Schermeyer, M.-T.; Seidel, J.; Hubbuch, J.
2016. International journal of pharmaceutics, 505 (1-2), 246–254. doi:10.1016/j.ijpharm.2016.03.047

Squeeze flow rheometry as a novel tool for the characterization of highly concentrated protein solutions
Schermeyer, M.-T.; Sigloch, H.; Bauer, K. C.; Oelschlaeger, C.; Hubbuch, J.
2016. Biotechnology and Bioengineering, 113 (3), 576–587. doi:10.1002/bit.25834

Advances in inline quantification of co-eluting proteins in chromatography: Process-data-based model calibration and application towards real-life separation issues
Brestrich, N.; Sanden, A.; Kraft, A.; McCann, K.; Bertolini, J.; Hubbuch, J.
2015. Biotechnology and Bioengineering / Symposium, 112 (7), 1406–1416. doi:10.1002/bit.25546

High-throughput process development of purification alternatives for the protein avidin
Diederich, P.; Hoffmann, M.; Hubbuch, J.
2015. Biotechnology progress, 31 (4), 957–973. doi:10.1002/btpr.2104

Predicting recombinant protein expression experiments using molecular dynamics simulation
Schaller, A.; Connors, N. K.; Oelmeier, S. A.; Hubbuch, J.; Middelberg, A. P. J.
2015. Chemical engineering science, 121, 340–350. doi:10.1016/j.ces.2014.09.044

Cationic aerosol photopolymerization
Akguen, E.; Muntean, A.; Hubbuch, J.; Woerner, M.; Sangermano, M.
2015. Macromolecular materials and engineering, 300 (2), 136–139. doi:10.1002/mame.201400211

A comprehensive molecular dynamics approach to protein retention modeling in ion exchange chromatography
Lang, K. M. H.; Kittelinann, J.; Duerr, C.; Osberghaus, A.; Hubbuch, J.
2015. Journal of chromatography / A, 1381, 184–193. doi:10.1016/j.chroma.2015.01.018

Downstream processing of virus-like particles: Single-stage and multi-stage aqueous two-phase extraction
Effio, C. L.; Wenger, L.; Oetes, O.; Oelmeier, S. A.; Kneusel, R.; Hubbuch, J.
2015. Journal of chromatography / A, 1383, 35–46. doi:10.1016/j.chroma.2015.01.007

Simulating and Optimizing Preparative Protein Chromatography with ChromX
Hahn, T.; Huuk, T.; Heuveline, V.; Hubbuch, J.
2015. Journal of chemical education, 92 (9), 1497–1502. doi:10.1021/ed500854a

Custom-tailored adsorbers: A molecular dynamics study on optimal design of ion exchange chromatography material
Lang, K. M. H.; Kittelmann, J.; Pilgram, F.; Osberghaus, A.; Hubbuch, J.
2015. Journal of chromatography / A, 1413, 60–67. doi:10.1016/j.chroma.2015.08.021

Systematic purification of salt-intolerant proteins by ion-exchange chromatography: The example of human α-galactosidase A
Baumann, P.; Osberghaus, A.; Hubbuch, J.
2015. Engineering in life sciences, 15 (2), 195–207. doi:10.1002/elsc.201400210

From osmotic second virial coefficient (B22) to phase behavior of a monoclonal antibody
Rakel, N.; Bauer, K. C.; Galm, L.; Hubbuch, J.
2015. Biotechnology progress, 31 (2), 438–451. doi:10.1002/btpr.2065

Computational study of elements of stability of a four-helix bundle protein biosurfactant
Schaller, A.; Connors, N. K.; Dwyer, M. D.; Oelmeier, S. A.; Hubbuch, J.; Middelberg, A. P. J.
2015. Journal of computer aided molecular design, 29 (1), 47–58. doi:10.1007/s10822-014-9803-6

High-throughput micro-scale cultivations and chromatography modeling: Powerful tools for integrated process development
Baumann, P.; Hahn, T.; Hubbuch, J.
2015. Biotechnology and Bioengineering / Symposium, 112 (10), 2123–2133. doi:10.1002/bit.25630

Robust high-throughput batch screening method in 384-well format with optical in-line resin quantification
Kittelmann, J.; Ottens, M.; Hubbuch, J.
2015. Journal of chromatography / B, 988, 98–105. doi:10.1016/j.jchromb.2015.02.028

Influence of macromolecular precipitants on phase behavior of monoclonal antibodies
Rakel, N.; Galm, L.; Bauer, K. C.; Hubbuch, J. J.
2015. Biotechnology progress, 31 (1), 145–153. doi:10.1002/btpr.2027

Effect of lysozyme solid-phase PEGylation on reaction kinetics and isoform distribution
Maiser, B.; Baumgartner, K.; Dismer, F.; Hubbuch, J.
2015. Journal of chromatography / B, 1002, 313–318. doi:10.1016/j.jchromb.2015.08.027

Integrated development of up- and downstream processes supported by the Cherry-Tag™ for real-time tracking of stability and solubility of proteins
Baumann, P.; Bluthardt, N.; Renner, S.; Burghardt, H.; Osberghaus, A.; Hubbuch, J.
2015. Journal of biotechnology, 200, 27–37. doi:10.1016/j.jbiotec.2015.02.024

Manipulation of lysozyme phase behavior by additives as function of conformational stability
Galm, L.; Morgenstern, J.; Hubbuch, J.
2015. International journal of pharmaceutics, 494 (1), 370–380. doi:10.1016/j.ijpharm.2015.08.045

Next generation vaccines and vectors: Designing downstream processes for recombinant protein-based virus-like particles
Effio, C. L.; Hubbuch, J.
2015. Biotechnology journal, 10 (5), 715–727. doi:10.1002/biot.201400392

Influence of binding pH and protein solubility on the dynamic binding capacity in hydrophobic interaction chromatography
Baumann, P.; Baumgartner, K.; Hubbuch, J.
2015. Journal of Chromatography A, 1396, 77–85. doi:10.1016/j.chroma.2015.04.001

Non-invasive high throughput approach for protein hydrophobicity determination based on surface tension
Amrhein, S.; Bauer, K. C.; Galm, L.; Hubbuch, J.
2015. Biotechnology and Bioengineering, 112 (12), 2485–2494. doi:10.1002/bit.25677

Automatisiertes Mikrofluidic-System mit optischem Aufbau zur Untersuchung von Peptid-Antikörper-Wecselwirkungen im Arrayformat
Weber, L. K.; Fischer, A.; Soehindrijo, M.; Förtsch, T. C.; Bojncic-Kninski, C.; Althuon, D.; Striffler, J.; Löffler, F. F.; Breitling, F.; Hubbuch, J.; Nesterov-Müller, A.
2015. MikroSystemTechnik Kongress 2015, Karlsruhe, 26.-28.Oktober 2015

Automatisiertes Mikrofluidic-System mit optischem Aufbau zur Untersuchung von Peptid-Antikörper-Wecselwirkungen im Arrayformat
Weber, L. K.; Fischer, A.; Soehindrijo, M.; Förtsch, T. C.; Bojncic-Kninski, C.; Althuon, D.; Striffler, J.; Löffler, F. F.; Breitling, F.; Hubbuch, J.; Nesterov-Müller, A.
2015. MikroSystemTechnik Kongress 2015, Karlsruhe, 26.-28.Oktober 2015, 120–122, VDE Verlag

Light extinction and scattering by agarose based resin beads and applications in high-throughput screening
Kittelmann, J.; Hämmerling, F.; Ebeler, M.; Hubbuch, J.
2015. Journal of chromatography / A, 1397, 52–58. doi:10.1016/j.chroma.2015.04.013

Cell-free expression of recombinant antigens of Borrelia burgdorferi and microarray-based multiplex detection using different patient sera
Richter, C.; Konstantinidis, K.; Asen, I.; Kneusel, R.; Hubbuch, J.
2014. Engineering in life sciences, 14 (4), 399–408. doi:10.1002/elsc.201300109

Automated measurement of apparent protein solubility to rapidly assess complex parameter interactions
Berg, A.; Schuetz, M.; Dismer, F.; Hubbuch, J.
2014. Food and bioproducts processing, 92 (2), 133–142. doi:10.1016/j.fbp.2013.10.002

High-throughput characterization of an insect cell-free expression
Richter, C.; Bickel, F.; Osberghaus, A.; Hubbuch, J.
2014. Engineering in life sciences, 14 (4), 409–417. doi:10.1002/elsc.201300118

Characterization of aqueous two phase systems by combining lab-on-a-chip technology with robotic liquid handling stations
Amrhein, S.; Schwab, M.-L.; Hoffmann, M.; Hubbuch, J.
2014. Journal of chromatography / A, 1367, 68–77. doi:10.1016/j.chroma.2014.09.042

Optimization of random PEGylation reactions by means of high throughput screening
Maiser, B.; Dismer, F.; Hubbuch, J.
2014. Biotechnology & bioengineering, 111 (1), 104–114. doi:10.1002/bit.25000

Perspectives of aerosol-photopolymerization: Organic-inorganic hybrid nanoparticles
Akgün, E.; Hubbuch, J.; Wörner, M.
2014. Colloid & polymer science, 292 (5), 1241–1247. doi:10.1007/s00396-014-3175-2

Perspectives of aerosol-photopolymerization: Nanostructured polymeric particles
Akgün, E.; Hubbuch, J.; Wörner, M.
2014. Macromolecular materials and engineering, 299 (11), 1316–1328. doi:10.1002/mame.201400032

Moving through three-dimensional phase diagrams of monoclonal antibodies
Rakel, N.; Baum, M.; Hubbuch, J.
2014. Biotechnology progress, 30 (5), 1103–1113. doi:10.1002/btpr.1947

Molecular dynamics simulations approach for the characterization of peptides with respect to hydrophobicity
Amrhein, S.; Oelmeier, S. A.; Dismer, F.; Hubbuch, J.
2014. The journal of physical chemistry <Washington, DC> / B, 118 (7), 1707–1714. doi:10.1021/jp407390f

Adjoint-based estimation and optimization for column liquid chromatography models
Hahn, T.; Sommer, A.; Osberghaus, A.; Heuveline, V.; Hubbuch, J.
2014. Computers & chemical engineering, 64, 41–54. doi:10.1016/j.compchemeng.2014.01.013

Defined polymer shells on nanoparticles via a continuous aerosol-based process
Sigmund, S.; Akgün, E.; Meyer, J.; Hubbuch, J.; Wörner, M.; Kasper, G.
2014. Journal of Nanoparticle Research, 16 (8), 2533. doi:10.1007/s11051-014-2533-9

Determination of protein phase diagrams by microbatch experiments: Exploring the influence of precipitants and pH
Baumgartner, K.; Galm, L.; Nötzold, J.; Sigloch, H.; Morgenstern, J.; Schleining, K.; Suhm, S.; Oelmeier, S. A.; Hubbuch, J.
2014. International Journal of Pharmaceutics, 479 (1), 28–40. doi:10.1016/j.ijpharm.2014.12.027

A tool for selective inline quantification of co-eluting proteins in chromatography using spectral analysis and partial least squares regression
Brestrich, N.; Briskot, T.; Osberghaus, A.; Hubbuch, J.
2014. Biotechnology and Bioengineering, 111 (7), 1365–373. doi:10.1002/bit.25194

Model-based integrated optimization and evaluation of a multi-step ion exchange chromatography
Huuk, T.; Hahn, T.; Osberghaus, A.; Hubbuch, J.
2014. Separation and Purification Technology, 136, 207–222. doi:10.1016/j.seppur.2014.09.012

Meeting Report VLPNPV: Session 10: Virus-like particle and nano-particle vaccines
Ladd Effio, C.; Hubbuch, J.
2014. Human vaccines & immunotherapeutics, 10 (10), 3080–3082. doi:10.4161/hv.29840

Untersuchung des Einflusses des Beschichtungsprozesses auf die Aktivität eines eingebetteten Enzyms in gedruckten Biosensoren
Riegel, A. L.; Borzenkova, N.; Hubbuch, J.; Scharfer, P.; Schabel, W.
2014. Chemie - Ingenieur - Technik, 86 (9), 1477. doi:10.1002/cite.201450411

Soluble full-length expression and characterization of snRNP protein U1-68/70 K
Richter, C.; Simon, T.; Asen, I.; Brenn-Weiss, G.; Hubbuch, J.
2014. Protein expression and purification, 104, 65–70. doi:10.1016/j.pep.2014.08.009

Microfluidics on liquid handling stations (μF-on-LHS): A new industry-compatible microfluidic platform
Kittelmann, J.; Radtke, C. P.; Waldbaur, A.; Neumann, C.; Hubbuch, J.; Rapp, B. E.
2014. Microfluidics, BioMEMS, and Medical Microsystems XII : Proceedings of Photonics West, San Francisco, Calif., February 2-4, 2014. Ed.: B. L. Gray, Article no 89760G, Society of Photo-optical Instrumentation Engineers (SPIE). doi:10.1117/12.2044665

Microfluidics on liquid handling stations (μF-on-LHS): A new industry-compatible microfluidic platform
Waldbaur, A.; Kittelmann, J.; Radtke, C. P.; Hubbuch, J.; Rapp, B. E.
2014. Microfluidics, BioMEMS, and Medical Microsystems XII, San Francisco, Calif., February 2-4, 2014

Selective high throughput protein quantification based on UV absorption spectra
Hansen, S. K.; Jamali, B.; Hubbuch, J.
2013. Biotechnology & bioengineering, 110 (2), 448–460. doi:10.1002/bit.24712

Accurate retention time determination of co-eluting proteins in analytical chromatography by means of spectral data
Dismer, F.; Hansen, S.; Oelmeier, S. A.; Hubbuch, J.
2013. Biotechnology & bioengineering, 110 (3), 683–693. doi:10.1002/bit.24738

Systematic generation of buffer systems for pH gradient ion exchange chromatography and their application
Kröner, F.; Hubbuch, J.
2013. Journal of chromatography / A, 1285, 78–87. doi:10.1016/j.chroma.2013.02.017

Self-interaction chromatography in pre-packed columns: A critical evaluation of self-interaction chromatography methodology to determine the second virial coefficient
Rakel, N.; Schleining, K.; Dismer, F.; Hubbuch, J.
2013. Journal of chromatography / A, 1293, 75–84. doi:10.1016/j.chroma.2013.03.077

Molecular dynamics simulations of aqueous two-phase systems: Understanding phase formation and protein partitioning
Dismer, F.; Alexander Oelmeier, S.; Hubbuch, J.
2013. Chemical engineering science, 96, 142–151. doi:10.1016/j.ces.2013.03.020

Perspectives of aerosol-photopolymerization: Nanoscale polymer particles
Akgün, E.; Hubbuch, J.; Wörner, M.
2013. Chemical engineering science, 101, 248–252. doi:10.1016/j.ces.2013.06.010

Analytical characterization of complex, biotechnological feedstocks by pH gradient ion exchange chromatography for purification process development
Kröner, F.; Hanke, A. T.; Nfor, B. K.; Pinkse, M. W. H.; Verhaert, P. D. E. M.; Ottens, M.; Hubbuch, J.
2013. Journal of chromatography / A, 1311, 55–64. doi:10.1016/j.chroma.2013.08.034

A high-throughput 2D-analytical technique to obtain single protein parameters from complex cell lysates for in silico process development of ion exchange chromatography
Kröner, F.; Elsäßer, D.; Hubbuch, J.
2013. Journal of chromatography / A, 1318, 84–91. doi:10.1016/j.chroma.2013.09.043

Alternative separation steps for monoclonal antibody purification: Combination of centrifugal partitioning chromatography and precipitation
Oelmeier, S.; Ladd Effio, C.; Hubbuch, J.
2013. Journal of Chromatography A, 1319, 118–126. doi:10.1016/j.chroma.2013.10.043

Evaluation of PEG/phosphate aqueous two-phase systems for the purification of the chicken egg white protein avidin by using high-throughput techniques
Diederich, P.; Amrhein, S.; Hämmerling, F.; Hubbuch, J.
2013. Chemical Engineering Science, 104, 945–956. doi:10.1016/j.ces.2013.10.008

Microfluidics on liquid handling stations (μF-on-LHS): an industry compatible chip interface between microfluidics and automated liquid handling stations
Waldbaur, A.; Kittelmann, J.; Radtke, C. P.; Hubbuch, J.; Rapp, B. E.
2013. Lab on a Chip - Miniaturisation for Chemistry and Biology, 13 (12), 2337–2343. doi:10.1039/c3lc00042g

Molecular dynamics simulations on aqueous two-phase systems - Single PEG-molecules in solution
Oelmeier, S. A.; Dismer, F.; Hubbuch, J.
2012. BMC Biophysics, 5 (1), 14. doi:10.1186/2046-1682-5-14

Application of genetic algorithms and response surface analysis for the optimization of batch chromatographic systems
Treier, K.; Lester, P.; Hubbuch, J.
2012. Biochemical engineering journal, 63, 66–75. doi:10.1016/j.bej.2012.02.003

High-throughput methods for miniaturization and automation of monoclonal antibody purification processes
Treier, K.; Hansen, S.; Richter, C.; Diederich, P.; Hubbuch, J.; Lester, P.
2012. Biotechnology progress, 28 (3), 723–732. doi:10.1002/btpr.1533

Rapid quantification of protein-polyethylene glycol conjugates by multivariate evaluation of chromatographic data
Hansen, S. K.; Maiser, B.; Hubbuch, J.
2012. Journal of chromatography / A, 1257, 41–47. doi:10.1016/j.chroma.2012.07.089

Editorial: High-throughput process development
Hubbuch, J.
2012. Biotechnology journal, 7 (10), 1185–1185. doi:10.1002/biot.201200333

Development and characterization of an automated high throughput screening method for optimization of protein refolding processes
Berg, A.; Oelmeier, S. A.; Kittelmann, J.; Dismer, F.; Hubbuch, J.
2012. Journal of separation science, 35 (22), 3149–3159. doi:10.1002/jssc.201200306

High-throughput screening-based selection and scale-up of aqueous two-phase systems for pDNA purification
Wiendahl, M.; Oelmeier, S. A.; Dismer, F.; Hubbuch, J.
2012. Journal of separation science, 35 (22), 3197–3207. doi:10.1002/jssc.201200310

Model-integrated process development demonstrated on the optimization of a robotic cation exchange step
Osberghaus, A.; Drechsel, K.; Hansen, S.; Hepbildikler, S.; Nath, S.; Haindl, M.; Lieres, E. von; Hubbuch, J.
2012. Chemical Engineering Science, 76, 129–139. doi:10.1016/j.ces.2012.04.004

Examination of a genetic algorithm for the application in high-throughput downstream process development
Treier, K.; Berg, A.; Diederich, P.; Lang, K.; Osberghaus, A.; Dismer, F.; Hubbuch, J.
2012. Biotechnology Journal, 7 (10), 1203–1215. doi:10.1002/biot.201200145

Detection, quantification, and propagation of uncertainty in high-throughput experimentation by Monte Carlo methods
Osberghaus, A.; Baumann, P.; Hepbildikler, S.; Nath, S.; Haindl, M.; Lieres, E. von; Hubbuch, J.
2012. Chemical Engineering and Technology, 35 (8), 1456–1464. doi:10.1002/ceat.201100610

Determination of parameters for the steric mass action model - A comparison between experimental and modeling approaches
Osberghaus, A.; Hepbildikler, S.; Nath, S.; Haindl, M.; Lieres, E. von; Hubbuch, J.
2012. Journal of Chromatography A, 1233, 54–64. doi:10.1016/j.chroma.2012.02.004

Optimizing a chromatographic three component separation: A comparison of mechanistic and empiric modeling approaches
Osberghaus, A.; Hepbildikler, S.; Nath, S.; Haindl, M.; Lieres, E. von; Hubbuch, J.
2012. Journal of Chromatography A, 1237, 86–95. doi:10.1016/j.chroma.2012.03.029

High throughput screening based selection of phases for aqueous two-phase system-centrifugal partitioning chromatography of monoclonal antibodies
Oelmeier, S.; Ladd Effio, C.; Hubbuch, J.
2012. Journal of Chromatography A, 1252, 104–114. doi:10.1016/j.chroma.2012.06.075

Isoform separation and binding site determination of mono-PEGylated lysozyme with pH gradient chromatography
Maiser, B.; Kröner, F.; Dismer, F.; Brenner-Weiß, G.; Hubbuch, J.
2012. Journal of Chromatography A, 1268, 102–108. doi:10.1016/j.chroma.2012.10.047

A sub-two minutes method for monoclonal antibody-aggregate quantification using parallel interlaced size exclusion high performance liquid chromatography
Diederich, P.; Hansen, S. K.; Oelmeier, S. A.; Hubbuch, J.
2011. Journal of Chromatography A, 1218 (50), 9010–9018. doi:10.1016/j.chroma.2011.09.086

A label-free methodology for selective protein quantification by means of absorption measurements
Hansen, S. K.; Skibsted, E.; Staby, A.; Hubbuch, J.
2011. Biotechnology and Bioengineering, 108 (11), 2661–2669. doi:10.1002/bit.23229

Application of an aqueous two-phase systems high-throughput screening method to evaluate mAb HCP separation
Oelmeier, S. A.; Dismer, F.; Hubbuch, J.
2010. Biotechnology and Bioengineering, 108 (1), 69–81. doi:10.1002/bit.22900

3D structure-based protein retention prediction for ion-exchange chromatography
Dismer, F.; Hubbuch, J.
2010. Journal of Chromatography A, 1217 (8), 1343–1354. doi:10.1016/j.chroma.2009.12.061

Advances in resins for ion-exchange chromatography
Staby, A.; Nielsen, J.; Krarup, J.; Wiendahl, M.; Hansen, T. B.; Kidal, S.; Hubbuch, J.; Mollerup, J.
2009. Advances in chromatography, 47, 193–245

A novel method to evaluate protein solubility using a high throughput screening approach
Wiendahl, M.; Völker, C.; Husemann, I.; Krarup, J.; Staby, A.; Scholl, S.; Hubbuch, J.
2009. Chemical engineering science, 64 (17), 3778–3788. doi:10.1016/j.ces.2009.05.029

High Throughput Screening for the Design and Optimization of Chromatographic Processes: Automated Optimization of Chromatographic Phase Systems
Susanto, A.; Treier, K.; Knieps-Grünhagen, E.; Lieres, E. von; Hubbuch, J.
2009. Chemical Engineering & Technology, 32 (1), 140–154. doi:10.1002/ceat.200800350

Rational and systematic protein purification process development: the next generation
Nfor, B. K.; Verhaert, P. D. E. M.; Wielen, L. A. M. van der; Hubbuch, J.; Ottens, M.
2009. Trends in Biotechnology, 27 (12), 673–679. doi:10.1016/j.tibtech.2009.09.002

High Throughput Screening for the Design and Optimization of Chromatographic Processes: Assessment of Model Parameter Determination from High Throughput Compatible Data
Susanto, A.; Knieps-Grünhagen, E.; Lieres, E. von; Hubbuch, J.
2008. Chemical Engineering & Technology, 31 (12), 1846–1855. doi:10.1002/ceat.200800457

Detailed analysis of membrane adsorber pore structure and protein binding by advanced microscopy
Wang, J.; Dismer, F.; Hubbuch, J.; Ulbricht, M.
2008. Journal of Membrane Science, 320 (1-2), 456–467. doi:10.1016/j.memsci.2008.04.039

Critical evaluation and comparison of fluid distribution systems for industrialscale expanded bed adsorption chromatography columns
Arpanaei, A.; Heebøll-Nielsen, A.; Hubbuch, J. J.; Thomas, O. R. T.; Hobley, T. J.
2008. Journal of Chromatography A, 1198-1199, 131–139. doi:10.1016/j.chroma.2008.05.044

High Throughput Screening for the Design and Optimization of Chromatographic Processes - Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies
Wiendahl, M.; Schulze Wierling, P.; Nielsen, J.; Fomsgaard Christensen, D.; Krarup, J.; Staby, A.; Hubbuch, J.
2008. Chemical Engineering & Technology, 31 (6), 893–903. doi:10.1002/ceat.200800167

Confocal laser scanning microscopy as an analytical tool in chromatographic research
Hubbuch, J.; Kula, M. R.
2008. Bioprocess and Biosystems Engineering, 31 (3), 241–259

Effects of ionic strength and mobile phase pH on the binding orientation of lysozyme on different ion-exchange adsorbents
Dismer, F.; Petzold, M.; Hubbuch, J.
2008. Journal of Chromatography A, 1194 (1), 11–21. doi:10.1016/j.chroma.2007.12.085

Bottleneck Downstream Processing Present and future challenges = Bottleneck Downstream Processing - Gegenwart und Zukünftige Herausforderungen
Hubbuch, J.; Karau, A.; Kaiser, K.
2007. Chemie - Ingenieur - Technik, 79 (9), 1270–1271

Comparison of chromatographic ion-exchange resins VI. Weak anion-exchange resins
Staby, A.; Jensen, R. H.; Bensch, M.; Hubbuch, J.; Dunweber, D. L.; Krarup, J.; Nielsen, J.; Lund, M.; Kidal, S.; Hansen, T. B.; Jensen, I. H.
2007. Journal of Chromatography A, 1164 (1-2), 82–94. doi:10.1016/j.chroma.2007.06.048

A novel two-zone protein uptake model for affinity chromatography and its application to the description of elution band profiles of proteins fused to a family 9 cellulose binding module affinity tag
Kavoosi, M.; Sanaie, N.; Dismer, F.; Hubbuch, J.; Kilburn, D. G.; Haynes, C. A.
2007. Journal of Chromatography A, 1160 (1-2), 137–149. doi:10.1016/j.chroma.2007.05.107

A novel approach to characterize the binding orientation of lysozyme on ion-exchange resins
Dismer, F.; Hubbuch, J.
2007. Journal of Chromatography A, 1149 (2), 312–320. doi:10.1016/j.chroma.2007.03.074

Investigation of pore diffusion hindrance of monoclonal antibody in hydrophobic interaction chromatography using confocal laser scanning microscopy
Susanto, A.; Herrmann, T.; Lieres, E. von; Hubbuch, J.
2007. Journal of Chromatography A, 1149 (2), 178–188. doi:10.1016/j.chroma.2007.03.002

Isolation and purification of biotechnological products
Hubbuch, J.; Kula, M. R.
2007. Journal of Non-Equilibrium Thermodynamics, 32, 99–127

High throughput screening of packed bed chromatography coupled with SELDI-TOF MS analysis: Monoclonal antibodies vs. host cell protein
Wierling, P. S.; Bogumil, R.; Knieps-Grunhagen, E.; Hubbuch, J.
2007. Biotechnology and Bioengineering, 98 (2), 440–450. doi:10.1002/bit.21399

Changes in retention behavior of fluorescently labeled proteins during ion-exchange chromatography caused by different protein surface labeling positions
Teske, C. A.; Simon, R.; Niebisch, A.; Hubbuch, J.
2007. Biotechnology and Bioengineering, 98 (1), 193–200. doi:10.1002/bit.21374

High throughput screening techniques in downstream processing: Preparation, characterization and optimisation of aqueous two phase systems
Bensch, M.; Selbach, B.; Hubbuch, J.
2007. Chemical Engineering Science, 62 (7), 2011–2021. doi:10.1016/j.ces.2006.12.053

Separation of genomic DNA, RNA and open circular plasmid DNA from supercoiled plasmid DNA by combining denaturation, selective renaturation and aqueous two phase extraction
Frerix, A.; Geilenkirchen, P.; Muller, M.; Kula, M. R.; Hubbuch, J.
2007. Biotechnology and Bioengineering, 96 (1), 57–66. doi:10.1002/bit.21166

Developing a Chromatographic Column Model for Bovine Serum Albumin on Strong Anion-Exchanger Source 30Q applying Confocal Laser Scanning Microscope
Susanto, A.; Wekenborg, K.; Hubbuch, J.; Schmidt-Traub, H.
2006. Journal of Chromatography A, 1137, 63–75. doi:10.1016/j.chroma.2006.10.004

A short-cut method for the correction of light attenuation influences in the experimental data obtained from confocal laser scanning microscopy
Susanto, A.; Herrmann, T.; Hubbuch, J.
2006. Journal of Chromatography A, 1136, 29–38. doi:10.1016/j.chroma.2006.09.053

Characterizing solute binding to macroporous ion exchange membrane adsorbers using confocal laser scanning microscopy
Wickramasinghe, S. R.; Carlson, J. O.; Teske, C.; Hubbuch, J.; Ulbricht, M.
2006. Journal of Membrane Science, 281 (1-2), 609–618. doi:10.1016/j.memsci.2006.04.032

Competitive adsorption of labeled and native protein in confocal laser scanning microscopy
Teske, C. A.; Lieres, E. von; Schröder, M.; Ladiwala, A.; Cramer, S. M.; Hubbuch, J. J.
2006. Biotechnology and Bioengineering, 95 (1), 58–66. doi:10.1002/bit.20940

Exploitation of coil-globule pDNA transition induced by small changes in temperature, pH salt and PEG compositions for directed partitioning in aqueous two phase systems
Frerix, A.; Schonewald, M.; Geilenkirchen, P.; Muller, M.; Kula, M. R.; Hubbuch, J.
2006. Langmuir, 22 (9), 4282–4290. doi:10.1021/la052745u

Generation of equally sized particles using solid-liquid suspensions
Herrmann, T.; Schroder, M.; Hubbuch, J.
2006. Biotechnology Progress, 22 (3), 914–918. doi:10.1021/bp050296i

The influence of homogenisation conditions on biomass-adsorbent interactions during ion-exchange expanded bed adsorption
Hubbuch, J. J.; Brixius, P. J.; Lin, D.-Q.; Mollerup, I.; Kula, M.-R.
2006. Biotechnology and Bioengineering, 94 (3), 543–553. doi:10.1002/bit.20850

Direct quantification of intra-particle protein diffusion in chromatographic media
Schroder, M.; Lieres, E. von; Hubbuch, J.
2006. Journal of Physical Chemistry B, 110 (3), 1429–1436. doi:10.1021/jp0542726

Automated microscale high-throughput screening for chromatography resins
Wierling, P. S.; Bensch, M.; Schroeder, T.; Hubbuch, J.
2005. Chemie - Ingenieur - Technik, 77 (8), 1240. doi:10.1002/cite.200590165

High throughput screening in downstream processing - Aqueous two phase extraction as a competitive initial purification step
Bensch, M.; Wierling, P. S.; Selbach, B.; Hubbuch, J.
2005. Chemie - Ingenieur - Technik, 77 (8), 1193. doi:10.1002/cite.200590260

High throughput screening of chromatographic phases for rapid process development
Bensch, M.; Wierling, P. S.; Lieres, E. von; Hubbuch, J.
2005. Chemical Engineering & Technology, 28 (11), 1274–1284. doi:10.1002/ceat.200500153

Scalable recovery of plasmid DNA based on aqueous two-phase separations
Frerix, A.; Muller, M.; Kula, M. R.; Hubbuch, J.
2005. Biotechnology and Applied Biochemistry, 42 (1), 57–66

Protein labelling effects in confocal laser scanning microscopy
Teske, C. A.; Schroeder, M.; Simon, R.; Hubbuch, J.
2005. Journal of Physical Chemistry B, 109 (28), 13811–13817. doi:10.1021/jp050713+

Integrated bioprocesses
Schugerl, K.; Hubbuch, J.
2005. Current Opinion in Microbiology, 8 (3), 294–300. doi:10.1016/j.mib.2005.01.002

Biochemical Engineering Aspects of Expanded Bed Adsorption
Hubbuch, J.; Thömmes, J.; Kula, M.-R.
2005. Advances in biochemical engineering, biotechnology, 92, 101–123

The influence of biomass on the hydrodynamic behaviour and stability of expanded beds
Lin, D.-Q.; Thömmes, J.; Kula, M.-R.; Hubbuch, J. J.
2004. Biotechnology and Bioengineering, 87 (3), 337–346. doi:10.1002/bit.20118

Superparamagnetic adsorbents for high-gradient magnetic fishing of lectins out of legume extracts
Heebøll-Nielsen, A.; Dalkiær, M.; Hubbuch, J. J.; Thomas, O. R. T.
2004. Biotechnology and Bioengineering, 87 (3), 311–323. doi:10.1002/bit.20116

Mechanism and dynamics of protein transport in chromatographic media studied by confocal laser scanning microscopy. Part 2: Impact on chromatographic separations
Hubbuch, J.; Linden, T.; Knieps, E.; Ljunglof, A.; Thommes, J.; Kula, M. R.
2003. Journal of Chromatography A, 1021 (1-2), 105–115. doi:10.1016/j.chroma.2003.08.092

Mechanism and dynamics of protein transport in chromatographic media studied by confocal laser scanning microscopy. Part 1: The interplay of sorbent structure and fluid phase conditions
Hubbuch, J.; Linden, T.; Knieps, E.; Ljunglof, A.; Thommes, J.; Kula, M. R.
2003. Journal of Chromatography A, 1021 (1-2), 93–104. doi:10.1016/j.chroma.2003.08.112

Biomass/adsorbent electrostatic interactions in expanded bed adsorption: a zeta potential study
Lin, D. Q.; Brixius, P. J.; Hubbuch, J. J.; Thommes, J.; Kula, M. R.
2003. Biotechnology and Bioengineering, 83 (2), 149–157. doi:10.1002/bit.10654

Dynamics of protein uptake within the adsorbent particle during packed bed chromatography
Hubbuch, J.; Linden, T.; Knieps, E.; Thommes, J.; Kula, M. R.
2002. Biotechnology and Bioengineering, 80 (4), 359–368. doi:10.1002/bit.10500

High gradient magnetic affinity separation of trypsin from porcine pancreatin
Hubbuch, J. J.; Thomas, O. R. T.
2002. Biotechnology and Bioengineering, 79 (3), 301–313. doi:10.1002/bit.10285

A New Fluid Distribution System for Scale-Flexible Expanded Bed Adsorption
Hubbuch, J. J.; Heeboll-Nielsen, A.; Hobley, T. J.; Thomas, O. R. T.
2002. Biotechnology and Bioengineering, 78 (1), 35–43. doi:10.1002/bit.10170

The Use of Ion-Selective Electrodes for Evaluating Residence Time Distributions in Expanded Bed Adsorption Systems
Fernandez-Lahore, H. M.; Lin, D. Q.; Hubbuch, J. J.; Kula, M. R.; Thommes, J.
2001. Biotechnology Progress, 17 (6), 1128–1136. doi:10.1021/bp010118z

High gradient magnetic separation versus expanded bed adsorption: A first principle comparison
Hubbuch, J. J.; Matthiesen, D. B.; Hobley, T. J.; Thomas, O. R. T.
2001. Bioseparation, 10 (1-3), 99112