Dr.-Ing. Stefan Oelmeier
- Post Doc
- office hours: Donnerstags 13:00-14:00 nach Anmeldung (Email oder ILIAS)
- room: R. 020
- phone: +49 721 608 46227
- fax: +49 721 608 46240
- stefan oelmeier ∂does-not-exist.kit edu
Fritz-Haber-Weg 2
76131 Karlsruhe
Resume and publications
Scientific CV
|
06/2012 - now |
PostDoc at the Karlsruhe Institute of Technology |
| 06/2008 - 06/2012 |
Graduate Student at the University of Karlsruhe |
|
07/2007 - 05/2008 |
Masterthesis „An evaluation of displacement chromatography for the manufacturing scale purification of a complex fusion protein“ Process Biochemistry Group, BiogenIdec Inc., San Diego, USA |
|
10/2005 - 06/2007 |
Study of „Biotechnology / molecular biotechnology“ M. Sc. at the RWTH Aachen University |
|
08/2005 - 10/2005 |
Internship „Characterisation of allergens transiently expressed in tabacco leaves“ Institute for molecular biotechnology at the RWTH Aachen University |
|
04/2005 - 07/2005 |
Bachelorthesis „Characterization of the promoter and first gene of the phe-operon of Geobacillus stearothermophilus“ Institute for biotechnology at the RWTH Aachen University |
|
10/2002 - 06/2005 |
Study of „Biotechnology / molecular biotechnology“ B.Sc. at the RWTH Aachen University |
Research Project
Current
Protein-protein interactions, focussed on protein phase diagrams, protein crystallization and precipitation, and properties of highly concentrated protein solution. Purification of large biological entities (mammalian cells, VLPs) using non-chromatographic processes. Molecular dynamics simutions of aqueous two-phase systems.
Dissertation
Evaluation of high-throughput screening methologies for the determination of solubility and partitioning in aqueous two-phase systems of proteins.
Publications
Poster
Stefan Oelmeier, Jürgen Hubbuch
Screening Aqueous Two Phase Systems for the Purification of Proteins using a Robotic Platform
15th International Conference on Biopartitioning and Purification (BPP)
Brunel University, Uxbridge, UK (14-19 June 2009)
Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch
Characterizing phase formation in aqueous two phase systems by molecular dynamics simulations
International Symposium on the Separation of Proteins, Peptides & Polynucleotides – Bologna – 2010
Stefan Oelmeier, Jürgen Hubbuch
Statistical Profling of an automated screening method in the case of an ATPS screening tool
1st International Conference on High-Throughput Process Development – Krakow – 2010
Florian Dismer, Stefan Oelmeier, Jürgen Hubbuch
Robotics aided solvatochromic characterization of PEG-PO4 ATPS
16th International Conference on BioPartitioning and Purifcation - Puerto Vallarta – 2011
Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch
Evaluation of Centrifugal Partitioning Chromatography for the purifcation of monoclonal antibodies
16th International Conference on BioPartitioning and Purifcation - Puerto Vallarta – 2011
Talks
Florian Dismer, Stefan Oelmeier, Jürgen Hubbuch
Gaining mechanistic understanding of aqueous two-phase systems for bioseparation
1st European Congress of Applied Biotechnology – Berlin – 2011
Stefan Oelmeier, Jürgen Hubbuch
High throughput analytics
BioProcess International conference – Long Beach, CA, USA – 2011
Stefan Oelmeier, Jürgen Hubbuch
Protein Partitioning in Aqueous Two Phase Systems: A Molecular Dynamics Approach
ACS Conference, April 2012, San Diego, USA
Oelmeier, SA, Dismer, F, Dieterle, M, Richter, M, Hubbuch, J
Process Development Balancing Solubility and Partitioning –Aqueous Two-Phase Extraction of Proteins
July 29.-August 3. 2012, Recovery of Biological Products 15, Stowe, USA
Diederich P., Hansen S.K., Oelmeier S.A., Hubbuch J.
MAb-Aggregate Analysis within 2 Minutes using Parallel-Interlaced HPLC
Bioprocessing Summit, August 20-23, Boston, MA,USA
Paper
Stefan Oelmeier, Florian Dismer, Jürgen Hubbuch (2011)
Application of an aqueous two-phase systems high-throughput screening method to evaluate mAb HCP separation.
Biotechnology and bioengineering, 108(1), pp. 69-81 - link
Patrick Diederich, Sigrid K. Hansen, Stefan A. Oelmeier, Bianca Stolzenberger, Jürgen Hubbuch (2011)
A Sub-Two Minutes Method for Monoclonal Antibody-Aggregate Quantification using Parallel Interlaced Size Exclusion High Performance Liquid Chromatography
J Chrom A, 1218, pp. 9010-8 - link
Berg, A., Oelmeier, S. A., Kittelmann, J., Dismer, F., & Hubbuch, J. (2012)
Development and characterization of an automated high throughput screening method for optimization of protein refolding processes
Journal of separation science, 35(22), 3149–59 - link
Oelmeier, S. A., Dismer, F., & Hubbuch, J. (2012)
Molecular dynamics simulations on aqueous two-phase systems - Single PEG-molecules in solution
BMC biophysics, 5, 14 - link
Oelmeier, S. A., Ladd Effio, C., & Hubbuch, J. (2012)
High throughput screening based selection of phases for aqueous two-phase system-centrifugal partitioning chromatography of monoclonal antibodies
Journal of chromatography. A, 1252, 104–14 - link
Wiendahl, M., Oelmeier, S. A., Dismer, F., & Hubbuch, J. (2012)
High-throughput screening-based selection and scale-up of aqueous two-phase systems for pDNA purification
Journal of separation science, 35(22), 3197–207 - link
Hansen, S. K., & Oelmeier, S. A. (2012)
Meeting report: High-throughput process development--HTPD 2012: June 4-7, 2012, Avignon, France
Biotechnology journal, 7(10), 1189–91 - link
Dismer, F., Oelmeier, S. A., & Hubbuch, J. (2013) Molecular dynamics simulations of aqueous two-phase systems: Understanding phase formation and protein partitioning.
Chemical Engineering Science, 96, 142–151 - link
Dismer, F., Hansen, S., Oelmeier, S. A., & Hubbuch, J. (2013)
Accurate retention time determination of co-eluting proteins in analytical chromatography by means of spectral data.
Biotechnology and bioengineering, 110(3), 683–93 - link
Schulte, B., John, I., Simon, B., Brockmann, C., Oelmeier, S. A, Jahraus, B., Kirchgessner, H., Riplinger, S., Carlomagno, T., Wabnitz, G., Samstag, Y. (2013)
A reducing milieu renders cofilin insensitive to phosphatidylinositol 4,5-bisphosphate (PIP2) inhibition
The Journal of biological chemistry - link