Lebenslauf und Publikationen

Scientific CV

05/2015 - now	PhD student Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT)
01/2015 - 04/2015	Scientific assistant: Correlation of Salt Impact on Protein Phase Behavior in Relation to HIC Elution and Thermal Stability Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT)
05/2014 - 11/2014	Master thesis: Investigation of Salt Impact on Hydrophobicity and Solubility Behavior of Model Proteins in Dependence on pH Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT)
10/2013 - 03/2014	Internship at Novo Nordisk A/S Gentofte/Denmark Protein Phase Behavior with Focus on Isoelectic Precipitation
09/2012 - 12/2012	Student assistant: Upstream processing, optimisation of the fermentation Institute of Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology (KIT)
04/2012 - 08/2012	Bachelor thesis: Screening the yeast cells Candida shehatae, Cryptococcus podzolicus and Scheffersomyces stipitis for the production of single cell oil Institute of Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology (KIT)
10/2009 - 11/2014	Studies of Bioengineering at Karlsruhe Institute of Technology (KIT)

Research Projects

Protein phase behavior of biopharmaceutical proteins - Precipitation as unit operation in continuous downstream processing.

Publications

Paper

S. Großhans, M. Rüdt, A. Sanden, N. Brestrich, J. Morgenstern, S. Heissler, J. Hubbuch In-line Fourier-transform infrared spectroscopy as a versatile process analytical technology for preparative protein chromatography, Journal of Chromatography A (2017). https://doi.org/10.1016/j.chroma.2018.03.005

S. Großhans, G. Wang, C.Fischer, J. Hubbuch An integrated precipitation and ion-exchange chromatography process for antibody manufacturing: Process development strategy and continuous chromatography exploration, Journal of Chromatography A (2017). https://doi.org/10.1016/j.chroma.2017.12.013

K. Baumgartner, S. Großhans, J. Schütz, S. Suhm, J. Hubbuch Prediction of salt effects on protein phase behavior by HIC retention and thermal stability, Journal of pharmaceutical and biomedical analysis (2016), 128, 216-225, https://doi.org/10.1016/j.jpba.2016.04.040

I. Schulze, S. Hansen, S. Großhans, T. Rudszuck, K. Ochsenreither, C. Syldatk, A. Neumann, Characterization of newly isolated oleaginous yeasts-Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis, AMB Express 4 (2014), 1-11, http://dx.doi.org/10.1186/s13568-014-0024-0

Talks

S. Großhans, G. Wang, J. Hubbuch, Water on Hydrophobic Surfaces – Modeling of Precipitation, ACS Spring Meeting 2018, New Orleans, Louisiana, 18. March 2018

S. Großhans, G. Wang, P. Baumann, J. Hubbuch, Integrated precipitation and periodic counter-current chromatography process, ACS Spring Meeting 2017, San Francisco, California, 4th April 2017