CV and Publications

Scientific CV

09/2017 – now	Research associate at Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
09/2016 – 03/2017	Master thesis
	Alternatives to protein PEGylation Stability assessment of conjugated proteins
	Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
04/2014 – 09/2017	Research assistant
	Cultivation of E.Coli (BioLector System)
	Cell disruption (ultrasonic)
	Downstream processing (affinity chromatography, IEX, HIC)
	Analytics (capillary gel electrophoresis, SEC, MALDI-TOF, activity assays, OPTIM, NMR)
10/2015 – 02/2016	Internship at Synthon, Nijmegen
	Development of a chromatographic step to separate Antibody Drug Conjugate Species
11/2013 – 03/2014	Bachelor thesis
	Optimization of the production of soluble and native cherry-tag proteins using a high throughput approach
	Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
10/2010 – 03/2017	Studies of Bioengineering at Karlsruhe Institute of Technology (KIT)

Research Projects

Biopharmaceutical downstream processing, industry projects

Publications

Papers

P. Baumann, N. Bluthardt, S. Renner, H. Burghardt, A. Osberghaus, J. Hubbuch, Integrated development of up- and downstream processes supported by the Cherry-Tag for real-time tracking of stability and solubility of proteins, J. Biotechnol. 200 (2015) 27-37, http://dx.doi.org/10.1016/j.jbiotec.2015.02.024

Lebenslauf und Publikationen

Akademischer CV

09/2017 - jetzt	Ingenieur am Institut für Bio- und Lebensmitteltechnik, Molekulare Aufarbeitung von Bioprodukten, KIT
09/2016 – 03/2017	Masterarbeit
	Alternatives to protein PEGylation Stability assessment of conjugated proteins
	Institut für Bio- und Lebensmitteltechnik, Molekulare Aufarbeitung von Bioprodukten, KIT
04/2014 – 09/2017	Wissenschaftliche Hilfskraft
	Kultivierung von E.Coli (BioLector System)
	Zellaufschluss (Ultraschall)
	Aufreinigung (Affinitätschromatographie, IEX, HIC)
	Analytik (Kapillargelelektrophorese, SEC, MALDI-TOF, Aktivitätsassays, OPTIM, NMR)
10/2015 – 02/2016	Praktikum bei Synthon, Nijmegen
	Development of a chromatographic step to separate Antibody Drug Conjugate Species
11/2013 – 03/2014	Bachelorarbeit
	Optimization of the production of soluble and native cherry-tag proteins using a high throughput approach
	Institut für Bio- und Lebensmitteltechnik, Molekulare Aufarbeitung von Bioprodukten, KIT
10/2010 – 03/2017	Studium des Bioingenieurwesens am Karlsruher Institut für Technologie (KIT)

Forschungsbreich

Biopharmazeutische Aufreinigung, Industrieprojekte

M.Sc. Nicolai Bluthardt