CV and Publications

Scientific CV

02/2013 - now	PhD student at the Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
06/2012 - 11/2012	Thesis: “Comparison of experimental and model-based parameter determination for sorption modeling in hydrophobic interaction chromatography“, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
05/2011 - 02/2012	Student research project: “Determination of the optimum liquid level in the sump during flash-crystallization“, Institute of Thermal Process Engineering, Karlsruhe Institute of Technology (KIT)
10/2010 - 03/2011	Internship: “Optimization of sanitization conditions in protein A chromatography“, Genentech, Inc., San Francisco
04/2010 - 07/2010	Student assistant: Experimental parameter determination for mechanistic chromatography modelling, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)
10/2008 - 03/2010	Student assistant: Mentoring of tutorials for higher mathematics, Institute for Algebra and Geometry, Karlsruhe Institute of Technology (KIT)
10/2007 - 11/2012	Studies of Bioengineering, Karlsruhe Institute of Technology (KIT)

Research Projects

Model-based online process analysis for liquid chromatography applications

Publications

Paper

N. Brestrich, T. Briskot, A. Osberghaus, J. Hubbuch, A tool for selective inline quantification of co-eluting proteins in chromatography using spectral analysis and partial least squares regression, Biotechnology and Bioengineering 111 (2014) 1365-1373, http://onlinelibrary.wiley.com/doi/10.1002/bit.25194/abstract.

N. Brestrich, A. Sanden, A. Kraft, K. McCann, J. Bertolini, J. Hubbuch, Advances in inline quantification of co-eluting proteins in chromatography: Process-data-based model calibration and application towards real-life separation issues, Biotechnology and Bioengineering 112 (2015) 1406-1416, http://onlinelibrary.wiley.com/doi/10.1002/bit.25546/abstract.

N. Brestrich, T. Hahn, J. Hubbuch, Application of spectral deconvolution and inverse mechanistic modelling as a tool for root cause investigation in protein chromatography, Journal of Chromatography A 1437 (2016) 158-167, http://www.sciencedirect.com/science/article/pii/S0021967316300978.

Talks

N. Brestrich, S. Hansen, A. Osberghaus, J. Hubbuch, A label-free methodology for selective inline quantification of co-eluting proteins in chromatography by means of spectral data, Integrated Continuous Biomanufacturing, Castelldefels, Spain, October 22, 2013.

N. Brestrich, T. Briskot, A. Osberghaus, J. Hubbuch, A tool for selective inline quantification of co-eluting proteins in chromatography using spectral data, 10th International PhD Seminar on Chromatographic Separation Science, Egmond aan Zee, Netherlands, February 25, 2014.

N. Brestrich, T. Briskot, A. Osberghaus, J. Hubbuch, Advances in model-based inline peak deconvolution: Process-data-based model calibration and real-time pooling decisions, 15th International Symposium on Preparative and Industrial Chromatography and Allied Techniques, Basel, Switzerland, October 6, 2014.

N. Brestrich, A. Sanden, T. Briskot, J. Hubbuch, A PAT tool for chromatography enabling selective inline quantification and real-time pooling decisions, 11th International PhD Seminar on Chromatographic Separation Science, Sundern, Germany, February 17, 2015.

N. Brestrich, A. Sanden, T. Briskot, J. Hubbuch, A PAT tool for chromatography supporting batch and continuous processing, 249th ACS National Meeting and Exposition, Denver, USA, March 25, 2015.

N. Brestrich, A. Sanden, T. Briskot, J. Hubbuch, A PAT tool for chromatography supporting batch and continuous processing, Continuous Bioprocessing, Frankfurt, Germany, April 27, 2015.

N. Brestrich, A. Osberghaus, T. Hahn, J. Hubbuch, Model-based monitoring, control, and cause of error diagnostics of chromatography processes, 3rd European Congress of Applied Biotechnology, Nice, France, October 1, 2015.

N. Brestrich, T. Hahn, A. Sanden, T. Briskot, J. Hubbuch, PAT concepts for chromatography: Real-time monitoring, real-time control, and cause of error diagnostics, Integrated Continuous Biomanufacturing II, Berkeley, USA, November 4, 2015.

M. Rüdt, N. Brestrich, J. Hubbuch, Spectral deconvolution of chromatograms with PLS models and calibration-free methods, 251st ACS National Meeting and Exposition, San Diego, USA, March 14, 2015.

Poster

N. Brestrich, A. Sanden, K. McCann, J. Bertolini, J. Hubbuch, In-line detection of co-eluting proteins by spectral analysis during chromatographic purification of plasma protein: Application to PAT, Plasma Product Biotechnology Meeting, Chia Laguna, Italy, May 13, 2015.

M. Rüdt, N. Brestrich, J. Hubbuch, Spectral deconvolution of chromatograms without offline analytics, Integrated Continuous Biomanufacturing II, Berkeley, USA, November 4, 2015.

Awards

Emil Kirschbaum Award 2014, Karlsruhe Institute of Technology (KIT) Graduation Ceremony, June 2014.